MUCOSAL AND SYSTEMIC IMMUNE RESPONSES TO A RECOMBINANT PROTEIN EXPRESSED ON THE SURFACE OF THE ORAL COMMENSAL BACTERIUM STREPTOCOCCUS GORDONII AFTER ORAL COLONIZATION

Medaglini D.; King TP.; Fischetti VA.; Pozzi G.

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MUCOSAL AND SYSTEMIC IMMUNE RESPONSES TO A RECOMBINANT PROTEIN EXPRESSED ON THE SURFACE OF THE ORAL COMMENSAL BACTERIUM STREPTOCOCCUS GORDONII AFTER ORAL COLONIZATION

机译：口腔定殖后表达于口腔普通链球菌戈登氏菌表面的重组蛋白的黏膜和全身免疫反应

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To circumvent the need to engineer pathogenic microorganisms as live vaccine-delivery vehicles, a system was developed which allowed for the stable expression of a wide range of protein antigens on the surface of Grampositive commensal bacteria. The human oral commensal Streptococcus gordonii was engineered to surface express a 204-amino acid allergen from hornet venom (Ag5.2) as a fusion with the anchor region of the M6 protein of Streptococcus pyogenes. The immunogenicity of the h16-Ag5.2 fusion protein was assessed in mice inoculated orally and intranasally with a single dose of recombinant bacteria, resulting in the colonization of the oral/pharyngeal mucosa for 10-11 weeks. A significant increase of Ag5.2-specific IgA with relation to the total IgA was detected in saliva and lung lavages when compared with mice colonized with wild-type S. gordonii. A systemic IgG response to Ag5.2 was also induced after oral colonization. Thus, recombinant Gram-positive commensal bacteria may be a safe and effective way of inducing a local and systemic immune response. [References: 35]

机译：为了避免将病原微生物改造为活疫苗递送载体的需要，开发了一种系统，该系统可在革兰氏阳性共生细菌表面稳定表达多种蛋白质抗原。工程改造人类口腔通用戈登链球菌，使其表面表达大黄蜂毒液（Ag5.2）的204个氨基酸变应原，与化脓链球菌M6蛋白的锚定区融合。在口服和鼻内接种单剂量重组细菌的小鼠中评估了h16-Ag5.2融合蛋白的免疫原性，导致口腔/咽粘膜定植10-11周。与定殖有野生沙门氏菌的小鼠相比，唾液和肺灌洗液中检测到Ag5.2特异性IgA相对于总IgA的显着增加。口服定植后，还诱导了对Ag5.2的全身性IgG应答。因此，重组革兰氏阳性共生细菌可能是诱导局部和全身免疫应答的安全有效方法。 [参考：35]

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来源
《Proceedings of the National Academy of Sciences of the United States of America》 |1995年第15期|p. 6868-6872|共5页
作者
Medaglini D.; King TP.; Fischetti VA.; Pozzi G.;
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作者单位

ROCKEFELLER UNIV BACTERIAL PATHOGENESIS & IMMUNOL LAB NEW YORK NY 10021 USA;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
Secretory iga; Oral vaccine; Vaccine vector; M protein; Gram positive; Secretory immunoglobulin-a; M6 protein; Salmonella-typhimurium; Vaccinia virus; Region; Induction; Sequence; Defense; Antigen; Sanguis;

机译：分泌型iga;口服疫苗;疫苗载体;M蛋白;革兰氏阳性;分泌型免疫球蛋白-a;M6蛋白;鼠伤寒沙门氏菌;牛痘病毒;区域;诱导;序列;防御;抗原;桑吉斯;

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机译：鼻腔接种表达肺炎球菌表面抗原A的重组乳酸菌对小鼠的全身和粘膜免疫应答的诱导和肺炎链球菌定植的减少。
2. Oral colonization and immune responses to Streptococcus gordonii expressing a pertussis toxin S1 fragment in mice [J] . Lee SF., Halperin SA., Wang H., FEMS Microbiology Letters . 2002,第2期

机译：在小鼠中对表达百日咳毒素S1片段的戈登链球菌的口腔定植和免疫反应
3. Systemic and mucosal immunity to respiratory syncytial virus induced by recombinant Streptococcus gordonii surface-displaying a domain of viral glycoprotein G. [J] . Falcone V, Mihm D, Neumann Haefelin D, FEMS immunology and medical microbiology . 2006,第1期

机译：重组戈登链球菌表面展示病毒糖蛋白G结构域诱导的呼吸道合胞病毒的全身和粘膜免疫。
4. Oral cochleates induce protective mucosal and systemic immune responses [C] . R.J.Mannino, S.Gould Fogerite International symposium on controlled release of bioactive materials;Consumer and diversified products conference . 2000

机译：口服耳蜗诱导保护性粘膜和全身免疫反应
5. Mucosal immunity to the commensal oral bacterium Streptococcus mitis biovar 1 in infants during the first year postpartum [D] . Wirth, Katherine Amy 2007

机译：产后第一年婴儿对口腔中常见的细菌微生物链球菌Biovar 1的粘膜免疫
6. Mucosal and systemic immune responses to a recombinant protein expressed on the surface of the oral commensal bacterium Streptococcus gordonii after oral colonization. [O] . D Medaglini, G Pozzi, T P King, 1995

机译：口腔定殖后对口腔共生细菌戈登氏链球菌表面表达的重组蛋白的粘膜和全身免疫反应。
7. Mucosal and systemic immune responses to a recombinant protein expressed on the surface of the oral commensal bacterium Streptococcus gordonii after oral colonization. [O] . Medaglini, D, Pozzi, G, King, T P, 1995

机译：口腔定殖后，对口腔共生细菌戈登氏链球菌表面表达的重组蛋白的粘膜和全身免疫反应。

MUCOSAL AND SYSTEMIC IMMUNE RESPONSES TO A RECOMBINANT PROTEIN EXPRESSED ON THE SURFACE OF THE ORAL COMMENSAL BACTERIUM STREPTOCOCCUS GORDONII AFTER ORAL COLONIZATION

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