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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >BIOSYNTHETIC TRANSPORT OF THE ASIALOGLYCOPROTEIN RECEPTOR H1 TO THE CELL SURFACE OCCURS VIA ENDOSOMES
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BIOSYNTHETIC TRANSPORT OF THE ASIALOGLYCOPROTEIN RECEPTOR H1 TO THE CELL SURFACE OCCURS VIA ENDOSOMES

机译：肠内糖酵素将H1的生物合成转运到细胞表面。

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摘要

Signals for endocytosis and for basolateral and lysosomal sorting are closely related in a number of membrane proteins, suggesting similar sorting mechanisms at the plasma membrane and in the trans-Golgi network (TGN). We tested the hypothesis that basolateral membrane proteins are transported to the cell surface via endosomes for the asialoglycoprotein receptor H1. This protein was tagged with a tyrosine sulfation site (H1(TS)) to allow specific labeling with [S-35]sulfate in the TGN. Madin-Darby canine kidney cells expressing H1(TS) were pulse-labeled and chased for a period of time insufficient for labeled H1(TS) to reach the cell surface. Upon homogenization and gradient centrifugation, fractions devoid of TGN were subjected to immunoisolation of compartments containing mannose 6-phosphate receptor, which served as an endosomal marker. H1(TS) in transit to the cell surface was efficiently coisolated, whereas a labeled secretory protein and free glycosaminoglycan chains were not. This indicates an indirect pathway for the asialoglycoprotein receptor to the plasma membrane via endosomes and has important implications for protein sorting in the TGN and endosomes. [References: 37]
机译：内吞信号以及基底外侧和溶酶体分选的信号在许多膜蛋白中密切相关，表明质膜和反高尔基体网络（TGN）的分选机制相似。我们测试了假说：基底膜蛋白通过去唾液酸糖蛋白受体H1的内体转运到细胞表面。将该蛋白标记有酪氨酸硫酸化位点（H1（TS）），以便在TGN中使用[S-35]硫酸盐进行特异性标记。对表达H1（TS）的Madin-Darby犬肾细胞进行脉冲标记，并追踪一段时间，以使标记的H1（TS）不能到达细胞表面。均质化和梯度离心后，将不含TGN的级分进行免疫分离，分离含有甘露糖6磷酸受体的内室，该受体用作内体标记。 H1（TS）转运到细胞表面的有效隔离，而标记的分泌蛋白和游离糖胺聚糖链则没有。这表明去唾液酸糖蛋白受体通过内体进入质膜的间接途径，对TGN和内体中的蛋白质分选具有重要意义。 [参考：37]

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