A DETERGENT-FREE METHOD FOR PURIFYING CAVEOLAE MEMBRANE FROM TISSUE CULTURE CELLS

Smart EJ.; Mineo C.; Anderson RGW.; Ying YS.

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A DETERGENT-FREE METHOD FOR PURIFYING CAVEOLAE MEMBRANE FROM TISSUE CULTURE CELLS

机译：一种无去垢剂的组织培养细胞纯化腔膜膜的方法

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Current methods for purifying caveolae from tissue culture cells take advantage of the Triton X-100 insolubility of this membrane domain. To circumvent the use of detergents, we have developed a method that depends upon the unique buoyant density of caveolae membrane. The caveolae fractions that we obtain are highly enriched in caveolin. As a consequence we are able to identify caveolae-associated proteins that had previously gone undetected. Moreover, resident caveolae proteins that are soluble in Triton X-100 are retained during the isolation. [References: 30]

机译：从组织培养细胞中纯化小窝的当前方法利用了该膜结构域的Triton X-100不溶性。为了避免使用洗涤剂，我们开发了一种方法，该方法取决于海绵膜的独特浮力密度。我们获得的小窝部分富含小窝蛋白。结果，我们能够鉴定以前未被发现的与小窝相关的蛋白。而且，在分离过程中保留了可溶于Triton X-100的驻留小窝蛋白。 [参考：30]

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《Proceedings of the National Academy of Sciences of the United States of America》 |1995年第22期|p. 10104-10108|共5页
作者
Smart EJ.; Mineo C.; Anderson RGW.; Ying YS.;
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作者单位

UNIV TEXAS SW MED CTR DEPT CELL BIOL & NEUROSCI DALLAS TX 75235 USA;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
G proteins; Epidermal growth factor receptor; Folate receptor; Gamma-subunits; Proteins; Receptor; Transport; Molecules; Surface; Pathway;

机译：G蛋白;表皮生长因子受体;叶酸受体;γ亚基;蛋白质;受体;转运;分子;表面;途径;

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1. Generally applicable methods to purify intracellular coccidia from cell cultures and to quantify purification efficacy using quantitative PCR [J] . Elsheikha HM, Rosenthal BM, Murphy AJ, Veterinary Parasitology . 2006,第3a4期

机译：通常用于从细胞培养物中纯化胞内球菌并使用定量PCR定量纯化功效的方法
2. Tissue-culture cell fractionation. Fractionation of membranes from tissue-culture cells homogenized by glycerol-induced lysis [J] . J K Sandall, J M Graham The biochemical journal . 1979,第1期

机译：组织培养细胞分级分离。甘油诱导的裂解使组织培养细胞膜均质分离
3. A NEW RAPID METHOD FOR THE ISOLATION OF SURFACE MEMBRANES FROM TISSUE CULTURE CELLS [J] . Peter Barland, Eleanor A. Schroeder Journal of cell biology . 1970,第3期

机译：从组织培养细胞中分离表面膜的新快速方法
4. A modification and purify method to culture the Schwann cells by adult SD rat [C] . Lao, J., Hung, Engineering in Medicine and Biology Society, 1998. Proceedings of the 20th Annual International Conference of the IEEE . 1998

机译：一种成年SD大鼠培养雪旺细胞的修饰纯化方法
5. Regulation of Limbal Stem Cells by Native Niche in HC-HA/PTX3 Purified From Amniotic Membrane [D] . Chen, Szu-Yu. 2018

机译：用羊膜纯化HC-HA / PTX3中的天然水质的峰干细胞的调节
6. A detergent-free method for purifying caveolae membrane from tissue culture cells. [O] . E J Smart, Y S Ying, C Mineo, 1995

机译：从组织培养细胞中纯化海绵膜的无洗涤剂方法。
7. A detergent-free method for purifying caveolae membrane from tissue culture cells. [O] . Smart, E J, Ying, Y S, Mineo, C, 1995

机译：从组织培养细胞中纯化海绵膜的无洗涤剂方法。
8. Intracellular Recordings from Pineal Cells in Tissue Culture: Membrane Properties and Response to Norepinephrine [R] . Freschi, J. E., Parfitt, A. G. 1986

机译：松果体细胞在组织培养中的细胞内记录：膜特性和对去甲肾上腺素的反应

A DETERGENT-FREE METHOD FOR PURIFYING CAVEOLAE MEMBRANE FROM TISSUE CULTURE CELLS

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