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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >LINEAGE SPECIFICATION OF NEURONAL PRECURSORS IN THE MOUSE SPINAL CORD
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LINEAGE SPECIFICATION OF NEURONAL PRECURSORS IN THE MOUSE SPINAL CORD

机译：小鼠脊髓中神经元前体的谱系指定

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We have investigated the differentiation potential of precursor cells within the developing spinal cord of mice and have shown that spinal cord cells from embryonic day 10 specifically give rise to neurons when plated onto an astrocytic monolayer, Ast-1. These neurons had the morphology of motor neurons and >83% expressed the motor neuron markers choline acetyltransferase, peripherin, calcitonin gene-related peptide, and L-14. By comparison, <10% of the neurons arising on monolayers of other neural cell lines or 3T3 fibroblasts had motor neuron characteristics. Cells derived from dorsal, intermediate, and ventral regions of the spinal cord all behaved similarly and gave rise to motor neuron-like cells when plated onto Ast-1. By using cells that expressed the lacZ reporter gene, it was shown that >93% of cells present on the Ast-1 monolayers were motor neuron-like. Time-lapse analysis revealed that the precursors on the Ast-1 monolayers gave rise to neurons either directly or following a single cell division. Together, these results indicate that precursors in the murine spinal cord can be induced to differentiate into the motor neuron phenotype by factors produced by Ast-1 cells, suggesting that a similar factor(s) produced by cells akin to Ast-1 may regulate motor neuron differentiation in vivo. [References: 33]
机译：我们研究了小鼠发育中的脊髓内前体细胞的分化潜能，并显示了从胚胎第10天起的脊髓细胞在平板接种到星形胶质细胞单层Ast-1上时特别产生神经元。这些神经元具有运动神经元的形态，> 83％的神经元表达运动神经元标志物胆碱乙酰转移酶，外周血蛋白，降钙素基因相关肽和L-14。相比之下，在其他神经细胞系或3T3成纤维细胞的单层上出现的神经元中，只有不到10％具有运动神经元特征。源自脊髓背侧，中间和腹侧区域的细胞的行为均相似，并且在接种到Ast-1上后会产生运动神经元样细胞。通过使用表达lacZ报告基因的细胞，Ast-1单层细胞中有93％以上的细胞是运动神经元样的。延时分析显示，Ast-1单层上的前体直接或在单个细胞分裂后产生神经元。总之，这些结果表明，由Ast-1细胞产生的因子可以诱导鼠脊髓中的前体分化为运动神经元表型，这表明类似于Ast-1的细胞产生的相似因子可以调节运动。体内神经元分化。 [参考：33]

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