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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >DETECTION OF CA2+ ENTRY THROUGH MECHANOSENSITIVE CHANNELS LOCALIZES THE SITE OF MECHANOELECTRICAL TRANSDUCTION IN HAIR CELLS
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DETECTION OF CA2+ ENTRY THROUGH MECHANOSENSITIVE CHANNELS LOCALIZES THE SITE OF MECHANOELECTRICAL TRANSDUCTION IN HAIR CELLS

机译：通过机械灵敏通道检测CA2 +进入使毛细胞中的机械电转移位置变得正常

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A hair cell, the sensory receptor of the internal ear, transduces mechanical stimuli into electrical responses. Transduction results from displacement of the hair bundle, a cluster of rod-shaped stereocilia extending from the cell's apical surface. Biophysical experiments indicate that, by producing shear between abutting stereocilia, a bundle displacement directly opens cation-selective transduction channels. Specific models of gating depend on the location of these channels, which has been controversial: although some physiological and immunocytochemical experiments have situated the transduction channels at the hair bundle's top, monitoring of fluorescence signals from the Ca2+ indicator fura-2 has instead suggested that Ca2+ traverses channels at the bundle's base. To examine the site of Ca2+ entry through transduction channels, we used laser-scanning confocal microscopy, with a spatial resolution of <1 mu m and a temporal resolution of <2 ms, to observe hair cells filled with the indicator fluo-3. An unstimulated hair cell showed a ''tip blush'' of enhanced fluorescence at the hair bundle's top, which we attribute to Ca2+ permeation through transduction channels open at rest. Upon mechanical stimulation, individual stereocilia displayed increased fluorescence that originated near their tips, then spread toward their bases. Our results confirm that mechanoelectrical transduction occurs near stereociliary tips. [References: 12]
机译：毛细胞（内耳的感觉受体）将机械刺激转换为电响应。转导是由发束的移位引起的，发束是从细胞的顶端表面延伸出来的一簇杆状立体纤毛。生物物理实验表明，通过在邻接的纤毛之间产生剪切力，束位移直接打开了阳离子选择性传导通道。特定的门控模型取决于这些通道的位置，这一直是有争议的：尽管一些生理和免疫细胞化学实验已将转导通道置于发束的顶部，但监测Ca2 +指示剂fura-2的荧光信号反而提示Ca2 +在包的底部遍历通道。为了检查Ca2 +通过转导通道进入的位置，我们使用激光扫描共聚焦显微镜（空间分辨率<1μm，时间分辨率<2 ms）观察充满指示器fluo-3的毛细胞。未经刺激的毛细胞在发束顶部显示出增强的荧光“尖端腮红”，这归因于Ca2 +通过静止时打开的转导通道渗透。在机械刺激下，单个的纤毛虫显示出增加的荧光，该荧光起源于其尖端附近，然后向其基部扩散。我们的结果证实了机电传导发生在睫状纤毛尖端附近。 [参考：12]

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