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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >ISOLATION, REGULATION, AND DNA-BINDING PROPERTIES OF THREE DROSOPHILA NUCLEAR HORMONE RECEPTOR SUPERFAMILY MEMBERS
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ISOLATION, REGULATION, AND DNA-BINDING PROPERTIES OF THREE DROSOPHILA NUCLEAR HORMONE RECEPTOR SUPERFAMILY MEMBERS

机译：三种果蝇核激素受体超家族成员的分离，调控及DNA结合特性

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We have designed a rapid cloning and screening strategy to identify new members of the nuclear hormone receptor superfamily that are expressed during the onset of Drosophila metamorphosis. Using this approach, we isolated three Drosophila genes, designated DHR38, DHR78, and DHR96. All three genes are expressed throughout third-instar larval and prepupal development. DHR38 is the Drosophila homolog of NGFI-B and binds specifically to an NGFI-B response element. DHR78 and DHR96 are orphan receptor genes. DHR78 is induced by 20-hydroxyecdysone (20E) in cultured larval organs, and its encoded protein binds to two AGGTCA half-sites arranged as either direct or palindromic repeats. DHR96 is also 20E-inducible, and its encoded protein binds selectively to the hsp27 20E response element. The 20E receptor can bind to each of the sequences recognized by DHR78 and DHR96, indicating that these proteins may compete with the receptor for binding to a common set of target sequences. [References: 38]
机译：我们设计了一种快速克隆和筛选策略，以鉴定果蝇变态发生期间表达的核激素受体超家族的新成员。使用这种方法，我们分离了三个果蝇基因，命名为DHR38，DHR78和DHR96。这三个基因在第三龄幼虫和and前发育过程中均表达。 DHR38是NGFI-B的果蝇同源物，可特异性结合NGFI-B应答元件。 DHR78和DHR96是孤儿受体基因。 DHR78是由20-羟基蜕皮激素（20E）在培养的幼体器官中诱导的，其编码的蛋白质与两个AGGTCA半位点结合，直接或回文重复排列。 DHR96也是20E诱导型的，其编码的蛋白质选择性地与hsp27 20E反应元件结合。 20E受体可以结合DHR78和DHR96识别的每个序列，表明这些蛋白质可以与受体竞争结合一组共同的靶序列。 [参考：38]

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