IDENTIFICATION OF THE GALACTOSE-ADHERENCE LECTIN EPITOPES OF ENTAMOEBA HISTOLYTICA THAT STIMULATE TUMOR NECROSIS FACTOR-ALPHA PRODUCTION BY MACROPHAGES

Seguin R; Mann BJ; Keller K; Chadee K

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IDENTIFICATION OF THE GALACTOSE-ADHERENCE LECTIN EPITOPES OF ENTAMOEBA HISTOLYTICA THAT STIMULATE TUMOR NECROSIS FACTOR-ALPHA PRODUCTION BY MACROPHAGES

机译：鉴定通过巨噬细胞刺激肿瘤坏死因子-α生产的恩氏单胞菌组织半乳糖粘附性卵磷脂表位

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The 170-kDa subunit of the galactose-adherence lectin (Gal-lectin) of Entamoeba histolytica mediates adherence to human colonic mucins and intestinal epithelium as a prerequisite to amebic invasion. The Gal-lectin is an immunodominant molecule and a protective antigen in the gerbil model of amebiasis. Tumor necrosis factor alpha (TNF-alpha) produced by activated macrophages enhances nitric oxide-dependent cytotoxicity in host defense against E. histolytica. The purpose of this study was to identify the Gal-lectin epitopes which stimulate TNF-alpha production by macrophages. Murine bone marrow-derived macrophages (BMMs) exposed to Gal-lectin (100-500 ng/ml) stimulated stable expression of TNF-alpha mRNA (8-fold increase) and TNF-alpha production similar to that of lipopolysaccharide-stimulated cells (100 ng/ml). Polyclonal anti-lectin serum specifically inhibited TNF-alpha mRNA induction in response to the Gal-lectin but not to lipopolysaccharide. Anti-lectin monoclonal antibodies 8C12, H85 and 1G7, which recognize nonoverlapping epitopes of the cysteine-rich region of the 170-kDa heavy subunit, inhibited both amebic adherence to mammalian cells and Gal-lectin-stimulated TNF-alpha mRNA expression by BMMs, but monoclonal antibody 7F4 did neither. As these inhibitory antibodies map to amino acids 596-1082 of the 170-kDa Gal-lectin, our results have identified the functional region that mediates amebic adherence and TNF-alpha mRNA induction in BMMs; thus, this region of the Gal-lectin is a subunit vaccine candidate.

机译：溶血变形杆菌的半乳糖粘附凝集素（Gal-lectin）的170 kDa亚基介导了对人类结肠粘蛋白和肠上皮的粘附，这是阿米巴侵袭的先决条件。 Gal-lectin在阿米巴病的沙鼠模型中是一种免疫优势分子和一种保护性抗原。活化的巨噬细胞产生的肿瘤坏死因子α（TNF-α）增强了宿主抗组织溶性大肠杆菌的一氧化氮依赖性细胞毒性。本研究的目的是鉴定刺激巨噬细胞刺激TNF-α产生的Gal-lectin表位。暴露于Gal-lectin（100-500 ng / ml）的鼠骨髓来源的巨噬细胞（BMM）刺激了TNF-αmRNA的稳定表达（增加了8倍）和TNF-α的产生，类似于脂多糖刺激的细胞（ 100 ng / ml）。多克隆抗凝集素血清特异性地响应于Gal-lectin而不是脂多糖抑制TNF-αmRNA的诱导。抗凝集素单克隆抗体8C12，H85和1G7能够识别170 kDa重亚基富含半胱氨酸的区域的非重叠表位，既能抑制阿米巴对哺乳动物细胞的依从性，又能抑制BMMs对Gal-lectin刺激的TNF-αmRNA表达，但是单克隆抗体7F4却没有。由于这些抑制性抗体定位于170 kDa Gal-lectin的氨基酸596-1082，因此我们的结果已经确定了介导BMM中阿米巴粘附和TNF-αmRNA诱导的功能区域。因此，Gal-lectin的这一区域是候选的亚单位疫苗。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1995年第26期|p.12175-12179|共5页
作者
Seguin R; Mann BJ; Keller K; Chadee K;
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作者单位

Chadee K, MCGILL UNIV, INST PARASITOL, MACDONALD CAMPUS, ST ANNE DE BELLEVUE, PQ H9X 3V9, CANADA;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
Amebic liver abscess; Binding lectin; Invitro; Trophozoites.; Immunization; Recognition; Interferon; Protection; Fractions.; Hamsters;

机译：阿米巴性肝脓肿;结合凝集素;体外;滋养体;免疫;识别;干扰素;保护;断裂;仓鼠;

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专利

1. The tumor necrosis factor alpha-stimulating region of galactose-inhibitable lectin of Entamoeba histolytica activates gamma interferon-primed macrophages for amebicidal activity mediated by nitric oxide. [J] . R Séguin, B J Mann, K Keller, Infection and immunity . 1997,第7期

机译：溶组织性变形杆菌的半乳糖抑制凝集素的肿瘤坏死因子α刺激区域激活一氧化氮介导的γ干扰素引发的巨噬细胞的杀螨活性。
2. Modulation of tumor necrosis factor production by macrophages in Entamoeba histolytica infection. [J] . W Wang, K Keller, K Chadee Infection and immunity . 1992,第8期

机译：溶血性变形杆菌感染中巨噬细胞对肿瘤坏死因子产生的调节。
3. Inhibitory effects of the stem bark of Catalpa ovata G. Don. (Bignoniaceae) on the productions of tumor necrosis factor-alpha and nitric oxide by the lipopolisaccharide-stimulated RAW 264.7 macrophages. [J] . Pae HO, Oh GS, Choi BM, Journal of Ethnopharmacology: An Interdisciplinary Journal Devoted to Bioscientific Research on Indigenous Drugs . 2003,第2a3期

机译：Catalpa ovata G. Don的茎皮的抑制作用。（洋紫薇）对脂多糖刺激的RAW 264.7巨噬细胞产生肿瘤坏死因子-α和一氧化氮的作用。
4. Pravastatin and Rosiglitazone May Attenuate Coronary Artery Disease by Decreasing Interferon-gamma, Tumor Necrosis Factor-alpha, Interleukin-6, Macrophage Chemotactic Protein-1, C-Reactive Protein, and Increasing Interleukin-4 [C] . L.G. Tan, S.A. Tan, L.S. Berk International Congress on Coronary Artery Disease . 2007

机译：普伐他汀和罗格列酮可以通过减少干扰素-γ，肿瘤坏死因子-α，白细胞介素-6，巨噬细胞趋化蛋白-1，C反应蛋白和增加白细胞介素-4
5. Bovine endothelial cells produce tumour necrosis factor-alpha, granulocyte macrophage colony stimulating factor and interleukin 1(beta) in response to Porphyromonas levii lipopolysaccharide [D] . Sparrow, David George 1998

机译：牛血管内皮细胞产生肿瘤坏死因子-α，粒细胞巨噬细胞集落刺激因子和白细胞介素1β响应卟啉单胞菌脂多糖
6. Identification of the galactose-adherence lectin epitopes of Entamoeba histolytica that stimulate tumor necrosis factor-alpha production by macrophages. [O] . R Séguin, B J Mann, K Keller, 1995

机译：鉴定半乳糖粘附凝集素表位的溶血性变形杆菌它刺激巨噬细胞产生肿瘤坏死因子-α。
7. Identification of the galactose-adherence lectin epitopes of Entamoeba histolytica that stimulate tumor necrosis factor-alpha production by macrophages. [O] . Séguin, R, Mann, B J, Keller, K, 1995

机译：鉴定半乳糖粘附凝集素表位的溶组织性变形杆菌，它刺激巨噬细胞产生肿瘤坏死因子-α。
8. Determination of a Unique Epitope Binding Site for a Complement-Lysis-EnhancingMonoclonal Antibody, 3D12, on the Galactose Adherence Lectin of Entamoeba histolytica, Using BIAcore [R] . Wood, S. J., Petri, W. 1992

机译：使用BIacore测定溶组织内阿米巴半乳糖粘附凝集素补体 - 裂解增强单克隆抗体3D12的独特表位结合位点

IDENTIFICATION OF THE GALACTOSE-ADHERENCE LECTIN EPITOPES OF ENTAMOEBA HISTOLYTICA THAT STIMULATE TUMOR NECROSIS FACTOR-ALPHA PRODUCTION BY MACROPHAGES

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