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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Proteome-wide protein interaction measurements of bacterial proteins of unknown function
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Proteome-wide protein interaction measurements of bacterial proteins of unknown function

机译：功能未知的细菌蛋白的全蛋白质组蛋白相互作用测量

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摘要

Despite the enormous proliferation of bacterial genome data, surprisingly persistent collections of bacterial proteins have resisted functional annotation. In a typical genome, roughly 30% of genes have no assigned function. Many of these proteins are conserved across a large number of bacterial genomes. To assign a putative function to these conserved proteins of unknown function, we created a physical interaction map by measuring biophysical interaction of these proteins. Binary protein-protein interactions in the model organism Streptococcus pneumoniae (TIGR4) are measured with a microfluidic high-throughput assay technology. In some cases, informatic analysis was used to restrict the space of potential binding partners. In other cases, we performed in vitro proteome-wide interaction screens. We were able to assign putative functions to 50 conserved proteins of unknown function that we studied with this approach.%Centre for Signalling Studies and Department of Microsystems Engineering, University of Freiburg 79110, Germany;Departments of Applied Physics and Bioengineering, Stanford University, Stanford, CA 94305,Howard Hughes Medical Institute, Stanford, CA 94605;Departments of Applied Physics and Bioengineering, Stanford University, Stanford, CA 94305,Howard Hughes Medical Institute, Stanford, CA 94605;
机译：尽管细菌基因组数据激增，但令人惊讶的细菌蛋白持久性集合却抵抗了功能注释。在典型的基因组中，大约30％的基因没有指定的功能。这些蛋白质中的许多蛋白质在大量细菌基因组中都是保守的。为了给这些功能未知的保守蛋白分配一个假定的功能，我们通过测量这些蛋白的生物物理相互作用来创建一个物理相互作用图。使用微流控高通量检测技术测量了模型生物体肺炎链球菌（TIGR4）中的二元蛋白质-蛋白质相互作用。在某些情况下，信息分析被用来限制潜在的有约束力的伴侣的空间。在其他情况下，我们进行了体外蛋白质组范围的相互作用筛选。我们能够将推定的功能分配给使用这种方法研究的50个未知功能的保守蛋白。％信号研究中心和微系统工程系，德国弗莱堡大学79110;德国斯坦福大学应用物理与生物工程系，斯坦福，CA 94305，霍华德·休斯医学院，斯坦福，CA 94605；斯坦福大学应用物理与生物工程系，斯坦福，CA 94305，霍华德·休斯医学院，斯坦福，CA 94605；

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  • 作者

    Matthias Meier; Rene V. Sit; Stephen R. Quake;

  • 作者单位

    Centre for Signalling Studies and Department of Microsystems Engineering, University of Freiburg 79110, Germany;

    Departments of Applied Physics and Bioengineering, Stanford University, Stanford, CA 94305,Howard Hughes Medical Institute, Stanford, CA 94605;

    Departments of Applied Physics and Bioengineering, Stanford University, Stanford, CA 94305,Howard Hughes Medical Institute, Stanford, CA 94605;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    high-throughput screening; unknown proteins;

    机译：高通量筛选未知蛋白质;

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