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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Minimization of thermodynamic costs in cancer cell invasion
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Minimization of thermodynamic costs in cancer cell invasion

机译:最小化癌细胞侵袭的热力学成本

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摘要

Metastasis, the truly lethal aspect of cancer, occurs when metastatic cancer cells in a tumor break through the basement membrane and penetrate the extracellular matrix. We show that MDA-MB-231 metastatic breast cancer cells cooperatively invade a 3D collagen matrix while following a glucose gradient. The invasion front of the cells is a dynamic one, with different cells assuming the lead on a time scale of 70 h. The front cell leadership is dynamic presumably because of metabolic costs associated with a long-range strain field that precedes the invading cell front which we have imaged using confocal imaging and marker beads imbedded in the collagen matrix. We suggest this could be a quantitative assay for an invasive phenotype tracking a glucose gradient and show that the invading cells act in a cooperative manner by exchanging leaders in the invading front.
机译:转移是癌症真正致命的方面,发生在肿瘤中的转移癌细胞穿过基底膜并穿透细胞外基质时。我们显示,MDA-MB-231转移性乳腺癌细胞在葡萄糖梯度作用下协同侵入3D胶原蛋白基质。细胞的入侵前沿是动态的,不同的细胞在70小时的时间尺度上处于领先地位。推测前细胞的领导是动态的,这是因为与远距离应变场相关的代谢成本,在我们使用共聚焦成像和嵌入胶原蛋白基质中的标记珠成像之前,远处的应变场就在入侵细胞前。我们建议这可能是一种跟踪葡萄糖梯度的侵袭性表型的定量检测方法,并表明通过交换入侵前沿的前导分子,入侵细胞以协同方式起作用。

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    Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100864, China;

    Physico-Chimie Curie, Unite Mixte de Recherche 168, Centre National de la Recherche Scientifique, 75005 Paris, France;

    Departments of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544;

    Departments of Physics, Princeton University, Princeton, NJ 08544;

    Departments of Electrical Engineering, Princeton University, Princeton, NJ 08544;

    Department of Integrated Mathematical Oncology, H. Lee Moffitt Cancer Center, Tampa, FL 33612;

    Department of Mathematics, Rutgers University, Piscataway, NJ 08854;

    Departments of Mechanical and Aerospace Engineering, Princeton University, Princeton, NJ 08544;

    Departments of Electrical Engineering, Princeton University, Princeton, NJ 08544;

    Department of Integrated Mathematical Oncology, H. Lee Moffitt Cancer Center, Tampa, FL 33612;

    Departments of Physics, Princeton University, Princeton, NJ 08544;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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