...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Reconciling molecular regulatory mechanisms with noise patterns of bacterial metabolic promoters in induced and repressed states
【24h】

Reconciling molecular regulatory mechanisms with noise patterns of bacterial metabolic promoters in induced and repressed states

机译：在诱导和抑制状态下，与细菌代谢启动子的噪声模式协调的分子调控机制

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Assessing gene expression noise in order to obtain mechanistic insights requires accurate quantification of gene expression on many individual cells over a large dynamic range. We used a unique method based on 2-photon fluorescence fluctuation microscopy to measure directly, at the single cell level and with single-molecule sensitivity, the absolute concentration of fluorescent proteins produced from the two Bacillus subtilis promoters that control the switch between glycolysis and gluconeogenesis. We quantified cell-to-cell variations in GFP concentrations in reporter strains grown on glucose or malate, including very weakly transcribed genes under strong catabolite repression. Results revealed strong transcriptional bursting, particularly for the glycolytic promoter. Noise pattern parameters of the two antagonistic promoters controlling the nutrient switch were differentially affected on glycolytic and gluconeogenic carbon sources, discriminating between the different mechanisms that control their activity. Our stochastic model for the transcription events reproduced the observed noise patterns and identified the critical parameters responsible for the differences in expression profiles of the promoters. The model also resolved apparent contradictions between in vitro operator affinity and in vivo represser activity at these promoters. Finally, our results demonstrate that negative feedback is not noise-reducing in the case of strong transcriptional bursting.
机译：评估基因表达噪声以获得机械学见解需要在大动态范围内准确定量许多个体细胞上的基因表达。我们使用了一种基于2光子荧光波动显微镜的独特方法，在单细胞水平上以单分子敏感性直接测量由两个枯草芽孢杆菌启动子产生的，控制糖酵解和糖异生之间转换的荧光蛋白的绝对浓度。 。我们对在葡萄糖或苹果酸上生长的报道菌株中GFP浓度的细胞间变化进行了定量，包括在强烈的分解代谢物抑制下转录非常弱的基因。结果显示强烈的转录爆发，特别是对于糖酵解启动子。控制营养转换的两个拮抗启动子的噪声模式参数在糖酵解和糖异生碳源上受到不同的影响，从而区分了控制其活性的不同机制。我们的转录事件的随机模型再现了观察到的噪声模式，并确定了导致启动子表达谱差异的关键参数。该模型还解决了体外操作员亲和力和这些启动子的体内阻遏物活性之间的明显矛盾。最后，我们的结果表明，在强烈的转录爆发情况下，负反馈不能降低噪音。

著录项

  • 来源
  • 作者

    Matthew L. Ferguson; Dominique Le Coq; Matthieu Jules; Stephane Aymerich; Ovidiu Radulescu; Nathalie Declerck; Catherine A. Royer;

  • 作者单位

    Centre de Biochimie Structurale, Institut National pour la Sante et la Recherche Medicale U554, Centre National pour la Recherche Scientifique Unite Mixte de Recherche 5048, Universite Montpellier 1 and 2, F-34090 Montpellier, France;

    Institut National pour la Recherche Agronomique, Unite Mixte de Recherche 1319 Micalis, F-78350 Jouy-en-Josas, France,Centre National pour la Recherche Scientifique, F-78350 Jouy-en-Josas, France,AgroParisTech, Unite Mixte de Recherche Micalis, F-78350 Jouy-en-Josas, France;

    Institut National pour la Recherche Agronomique, Unite Mixte de Recherche 1319 Micalis, F-78350 Jouy-en-Josas, France,AgroParisTech, Unite Mixte de Recherche Micalis, F-78350 Jouy-en-Josas, France;

    Institut National pour la Recherche Agronomique, Unite Mixte de Recherche 1319 Micalis, F-78350 Jouy-en-Josas, France,AgroParisTech, Unite Mixte de Recherche Micalis, F-78350 Jouy-en-Josas, France;

    Institut National pour la Recherche Agronomique Departement de Microbiologie, F-75338 Paris, France;

    Centre de Biochimie Structurale, Institut National pour la Sante et la Recherche Medicale U554, Centre National pour la Recherche Scientifique Unite Mixte de Recherche 5048, Universite Montpellier 1 and 2, F-34090 Montpellier, France,Institut National pour la Recherche Agronomique Departement de Microbiologie, F-75338 Paris, France;

    Centre de Biochimie Structurale, Institut National pour la Sante et la Recherche Medicale U554, Centre National pour la Recherche Scientifique Unite Mixte de Recherche 5048, Universite Montpellier 1 and 2, F-34090 Montpellier, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    B. subtilis; central carbon metabolism; promoter activity; stochastic gene expression; gene expression control;

    机译：枯草芽孢杆菌中央碳代谢启动子活性;随机基因表达;基因表达控制;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号