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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Nature of curvature coupling of amphiphysin with membranes depends on its bound density
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Nature of curvature coupling of amphiphysin with membranes depends on its bound density

机译：两栖生物与膜的曲率耦合性质取决于其结合密度

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摘要

Cells are populated by a vast array of membrane-binding proteins that execute critical functions. Functions, like signaling and intra-cellular transport, require the abilities to bind to highly curved membranes and to trigger membrane deformation. Among these proteins is amphiphysin 1, implicated in clathrin-mediated endo-cytosis. It contains a Bin-Amphiphysin-Rvs membrane-binding domain with an N-terminal amphipathic helix that senses and generates membrane curvature. However, an understanding of the parameters distinguishing these two functions is missing. By pulling a highly curved nanotube of controlled radius from a giant vesicle in a solution containing amphiphysin, we observed that the action of the protein depends directly on its density on the membrane. At low densities of protein on the nearly flat vesicle, the distribution of proteins and the mechanical effects induced are described by a model based on spontaneous curvature induction. The tube radius and force are modified by protein binding but still depend on membrane tension. In the dilute limit, when practically no proteins were present on the vesicle, no mechanical effects were detected, but strong protein enrichment proportional to curvature was seen on the tube. At high densities, the radius is independent of tension and vesicle protein density, resulting from the formation of a scaffold around the tube. As a consequence, the scaling of the force with tension is modified. For the entire density range, protein was enriched on the tube as compared to the vesicle. Our approach shows that the strength of curvature sensing and mechanical effects on the tube depends on the protein density.
机译：细胞由大量执行关键功能的膜结合蛋白组成。像信号传导和细胞内运输这样的功能需要结合到高度弯曲的膜并触发膜变形的能力。在这些蛋白中有两亲蛋白1，与网格蛋白介导的胞吞作用有关。它包含一个Bin-Amphphiphysin-Rvs膜结合结构域，该结构域具有N端两亲性螺旋，可感应并产生膜曲率。但是，缺少对区分这两个功能的参数的理解。通过在含有两性菌素的溶液中从一个巨大的囊泡中拉出一个半径受控的高度弯曲的纳米管，我们观察到该蛋白质的作用直接取决于其在膜上的密度。在接近平坦囊泡的低密度蛋白质中，蛋白质的分布和所诱导的机械效应由基于自发曲率诱导的模型描述。管半径和力通过蛋白质结合而改变，但仍取决于膜张力。在稀释极限中，当在囊泡上几乎没有蛋白质存在时，没有检测到机械作用，但是在试管上看到了与曲率成比例的强烈蛋白质富集。在高密度下，半径与张力和囊泡蛋白密度无关，这是由于在管周围形成支架所致。结果，随张力改变了力的比例。对于整个密度范围，与囊泡相比，蛋白质在管上富集。我们的方法表明，曲率检测的强度和对管的机械作用取决于蛋白质密度。

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  • 作者

    Benoit Sorre; Andrew Callan-Jones; John Manzi; Bruno Goud; Jacques Prost; Patricia Bassereau; Aurelien Roux;

  • 作者单位

    Institut Curie, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 168, Universite Pierre et Marie Curie, F-75248 Paris, France,Institut Curie, , Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, F-75248 Paris, France,Laboratory of Theoretical Condensed Matter Physics, The Rockefeller University, New York, NY 10065;

    Centre National de la Recherche Scientifique, Laboratoire Charles Coulomb, Universite Montpellier II, F-34095 Montpellier, France;

    Institut Curie, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 168, Universite Pierre et Marie Curie, F-75248 Paris, France;

    Institut Curie, , Centre National de la Recherche Scientifique, Unite Mixte de Recherche 144, F-75248 Paris, France;

    Institut Curie, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 168, Universite Pierre et Marie Curie, F-75248 Paris, France,Ecole Superieure de Physique et de ChimieIndustrielles, 10 rue Vauquelin, F-75231 Paris, France;

    Centre National de la Recherche Scientifique, Laboratoire Charles Coulomb, Universite Montpellier II, F-34095 Montpellier, France,Ecole Superieure de Physique et de ChimieIndustrielles, 10 rue Vauquelin, F-75231 Paris, France;

    Institut Curie, Centre National de la Recherche Scientifique, Unite Mixte de Recherche 168, Universite Pierre et Marie Curie, F-75248 Paris, France,Department of Biochemistry and National Centre of Competence in Research Chemical Biology, Science II, 30 quai Ernest Ansermet, CH-1211 Geneva, Switzerland;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    curvature-inducing; curvature-sensing; membrane nanotube; membrane physics;

    机译：曲率诱导曲率感应膜纳米管膜物理学;

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