...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The heterogeneous motility of the Lyme disease spirochete in gelatin mimics dissemination through tissue
【24h】

The heterogeneous motility of the Lyme disease spirochete in gelatin mimics dissemination through tissue

机译：明胶中的莱姆病螺旋体的异质运动模拟通过组织的传播

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Lyme disease spirochete Borrelia burgdorferi exists in nature in an enzootic cycle that involves the arthropod vector Ixodes scapularis and mammalian reservoirs. To disseminate within and between these hosts, spirochetes must migrate through complex, polymeric environments such as the basement membrane of the tick midgut and the dermis of the mammal. To date, most research on the motility of B. burgdorferi has been done in media that do not resemble the tissue milieus that B. burgdorferi encounter in vivo. Here we show that the motility of Borrelia in gelatin matrices in vitro resembles the pathogen's movements in the chronically infected mouse dermis imaged by intravital microscopy. More specifically, B. burgdorferi motility in mouse dermis and gelatin is heterogeneous, with the bacteria transitioning between at least three different motility states that depend on transient adhesions to the matrix. We also show that B. burgdorferi is able to penetrate matrices with pore sizes much smaller than the diameter of the bacterium. We find a complex relationship between the swimming behavior of B. burgdorferi and the rheological properties of the gelatin, which cannot be accounted for by recent theoretical predictions for microorganism swimming in gels. Our results also emphasize the importance of considering borrelial adhesion as a dynamic rather than a static process.
机译：莱姆病螺旋体伯氏疏螺旋体存在于自然界中，涉及一种节肢动物媒介肩x龙虾和哺乳动物水库。为了在这些宿主内和之间传播，螺旋体必须在复杂的聚合环境（例如migrate中肠的基底膜和哺乳动物的真皮）中迁移。迄今为止，大多数关于B. burgdorferi运动性的研究都是在与B. burgdorferi在体内遇到的组织环境不同的培养基中进行的。在这里，我们显示了在明胶基质中疏螺旋体的运动类似于活体显微镜下成像的慢性感染小鼠真皮中病原体的运动。更具体地说，小鼠真皮和明胶中的伯氏疏螺旋体运动性是异质的，细菌在至少三种不同的运动状态之间转变，这取决于对基质的瞬时粘附。我们还表明，B。burgdorferi能够穿透孔径小于细菌直径的基质。我们发现B. burgdorferi的游动行为与明胶的流变特性之间存在复杂的关系，这不能通过凝胶中的微生物游动的最新理论预测来解释。我们的研究结果还强调了将硼粘附力视为动态过程而非静态过程的重要性。

著录项

  • 来源
  • 作者

    Michael W. Harman; Star M. Dunham-Ems; Melissa J. Caimano; Alexia A. Belperron; Linda K. Bockenstedt; Henry C. Fu; Justin D. Radolf; Charles W. Wolgemuth;

  • 作者单位

    Departments of Medicine, Pediatrics, Genetics and Developmental Biology, and Immunology, University of Connecticut Health Center, Farmington, CT06030-3715,Department of Cell Biology and Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030-6406;

    Departments of Medicine, Pediatrics, Genetics and Developmental Biology, and Immunology, University of Connecticut Health Center, Farmington, CT06030-3715;

    Departments of Medicine, Pediatrics, Genetics and Developmental Biology, and Immunology, University of Connecticut Health Center, Farmington, CT06030-3715;

    Department of Internal Medicine, Yale University, New Haven, CT 06520-8031;

    Department of Internal Medicine, Yale University, New Haven, CT 06520-8031;

    Department of Mechanical Engineering, University of Nevada, Reno, NV 89509;

    Departments of Medicine, Pediatrics, Genetics and Developmental Biology, and Immunology, University of Connecticut Health Center, Farmington, CT06030-3715;

    Department of Cell Biology and Center for Cell Analysis and Modeling, University of Connecticut Health Center, Farmington, CT 06030-6406;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号