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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Aerobic kinetoplastid flagellate Phytomonas does not require heme for viability
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Aerobic kinetoplastid flagellate Phytomonas does not require heme for viability

机译：有氧运动性鞭毛鞭毛的拟南芥不需要血红素来维持生存力

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摘要

Heme is an iron-coordinated porphyrin that is universally essential as a protein cofactor for fundamental cellular processes, such as electron transport in the respiratory chain, oxidative stress response, or redox reactions in various metabolic pathways. Parasitic kinetoplastid flagellates represent a rare example of organisms that depend on oxidative metabolism but are heme auxotrophs.Here, we show that heme is fully dispensable for the survival of Phytomonas serpens, a plant parasite. Seeking to understand the metabolism of this heme-free eukaryote, we searched for hemecontaining proteins in its de novo sequenced genome and examined several cellular processes for which heme has so far been considered indispensable. We found that P. serpens lacks most of the known hemoproteins and does not require heme for electron transport in the respiratory chain, protection against oxidative stress, or desaturation of fatty acids. Although heme is still required for the synthesis of ergosterol, its precursor, lanosterol, is instead incorporated into the membranes of P. serpens grown in the absence of heme. In conclusion, P. serpens is a flagellate with unique metabolic adaptations that allow it to bypass all requirements for heme.
机译：血红素是铁配位的卟啉，普遍用作基本细胞过程的蛋白质辅因子，例如呼吸链中的电子运输，氧化应激反应或各种代谢途径中的氧化还原反应。寄生性动素体鞭毛是依赖于氧化代谢但属于血红素营养缺陷型的生物的罕见例子。在这里，我们证明血红素对于植物性寄生虫Phytomonas serpens的生存是完全不可或缺的。为了了解这种无血红素的真核生物的代谢，我们在其从头测序的基因组中搜索了含血红素的蛋白质，并研究了迄今为止被认为不可或缺的几种血红素细胞过程。我们发现，P。serpens缺乏大多数已知的血红素蛋白，不需要血红素来在呼吸链中进行电子转运，防止氧化应激或脂肪酸去饱和。虽然合成麦角固醇仍需要血红素，但其前体羊毛甾醇被并入在没有血红素的情况下生长的假单胞菌的膜中。总之，锯齿假单胞菌是鞭毛，具有独特的代谢适应性，可以绕过血红素的所有需求。

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  • 作者

    Ludek Kofeny; Roman Sobotka; Julie Kovafova; Anna Gnipova; Pavel Flegontov; Anton Horvath; Miroslav Obornik; Francisco J. Ayala; Julius Lukes;

  • 作者单位

    Biology Centre, Institute of Parasitology, Czech Academy of Sciences, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic Faculty of Science, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic;

    Faculty of Science, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic Institute of Microbiology, Czech Academy of Sciences, 379 81 Trebon, Czech Republic;

    Biology Centre, Institute of Parasitology, Czech Academy of Sciences, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic Faculty of Science, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic;

    Biology Centre, Institute of Parasitology, Czech Academy of Sciences, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovakia;

    Biology Centre, Institute of Parasitology, Czech Academy of Sciences, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic Faculty of Science, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic;

    Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovakia;

    Biology Centre, Institute of Parasitology, Czech Academy of Sciences, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic Faculty of Science, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic Institute of Microbiology, Czech Academy of Sciences, 379 81 Trebon, Czech Republic;

    Department of Ecology and Evolutionary Biology, University of California, Irvine, CA 92697;

    Biology Centre, Institute of Parasitology, Czech Academy of Sciences, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic Faculty of Science, University of South Bohemia, 370 05 Ceske Budejovice, Czech Republic;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    cytochromes; respiration; sterols; protist;

    机译：细胞色素;呼吸;固醇;原教徒;

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