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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Tomato immune receptor Ve1 recognizes effector of multiple fungal pathogens uncovered by genome and RNA sequencing
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Tomato immune receptor Ve1 recognizes effector of multiple fungal pathogens uncovered by genome and RNA sequencing

机译：番茄免疫受体Ve1识别基因组和RNA测序发现的多种真菌病原体的效应子

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摘要

Fungal plant pathogens secrete effector molecules to establish disease on their hosts, and plants in turn use immune receptors to try to intercept these effectors. The tomato immune receptor Ve1 governs resistance to race 1 strains of the soil-borne vascular wilt fungi Verticillium dahliae and Verticillium albo-atrum, but the corresponding Verticillium effector remained unknown thus far. By high-throughput population genome sequencing, a single 50-Kb sequence stretch was identified.that only occurs in race 1 strains, and subsequent transcriptome sequencing of Verticillium-'infected Nicotiana benthamiana plants revealed only a single highly expressed ORF in this region, designated Ave1 (for Avirulence on Ve1 tomato). Functional analyses confirmed that Avel activates Ve1-mediated resistance and demonstrated that Ave1 markedly contributes to fungal virulence, not only on tomato but also on Arabidopsis. interestingly, Ave1 is homologous to a widespread family of plant natriuretic peptides. Besides plants, homologous proteins were only found in the bacterial plant pathogen Xantho-monas axonopodis and the plant pathogenic fungi Colletotrichum higginsianum, Cercospora beticola, and Fusarium oxysporum f. sp. lycopersici. The distribution of Ave1 homologs, coincident with the presence of Ave1 within a flexible genomic region, strongly suggests that Verticillium acquired Ave1 from plants through horizontal gene transfer. Remarkably, by transient expression we show that also the Ave1 homologs from F. oxysporum and C. beticola can activate Vet-mediated resistance. In line with this observation, Ve1 was found to mediate resistance toward F. oxysporum in tomato, showing that this immune receptor is involved in resistance against multiple fungal pathogens.
机译：真菌植物病原体会分泌效应子分子以在其宿主上建立疾病，而植物又利用免疫受体来试图拦截这些效应子。番茄免疫受体Ve1决定了对土壤传播的青枯菌和黄萎病菌的第1种菌株的抗性，但至今尚不清楚相应的黄萎病效应子。通过高通量群体基因组测序，鉴定出仅在第1株菌株中发生的单个50 Kb序列延伸，随后对黄萎病菌感染的烟草本氏烟草植物进行的转录组测序显示在该区域中只有一个高度表达的ORF，称为Ave1（对于Ve1番茄无毒）。功能分析证实，Avel激活了Ve1介导的抗性，并证明Ave1不仅对番茄而且对拟南芥都有明显的真菌毒力。有趣的是，Ave1与广泛的植物利钠肽家族同源。除植物外，同源蛋白仅在细菌性植物病原体Xantho-monas axonopodis和植物病原真菌Colletotrichum higginsianum，Cercospora beticola和Fusarium oxysporum f中发现。 sp。 lycopersici。 Ave1同源物的分布与在灵活的基因组区域内Ave1的存在相吻合，强烈表明黄萎病菌通过水平基因转移从植物获得了Ave1。值得注意的是，通过瞬时表达，我们表明来自尖孢镰刀菌和锥状线虫的Ave1同源物也可以激活Vet介导的抗性。与该观察结果一致，发现Ve1介导了番茄中对尖孢镰刀菌的抗性，表明该免疫受体参与了对多种真菌病原体的抗性。

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  • 作者

    Ronnie de Jonge; H. Peter van Esse; Karunakaran Maruthachalam; Melvin D. Bolton; Parthasarathy Santhanam; Mojtaba Keykha Saber; Zhao Zhang; Toshiyuki Usami; Bart Lievens; Krishna V. Subbarao; Bart P. H. J. Thomma;

  • 作者单位

    Laboratory of Phytopathology, Wageningen University, 6708 PB Wageningen, The Netherlands;

    Laboratory of Phytopathology, Wageningen University, 6708 PB Wageningen, The Netherlands;

    Department of Plant Pathology, University of California, Davis, CA 95616;

    Agricultural Research Service, Northern Crop Science Laboratory, US Department of Agriculture, Fargo, ND 58102;

    Laboratory of Phytopathology, Wageningen University, 6708 PB Wageningen, The Netherlands;

    Laboratory of Phytopathology, Wageningen University, 6708 PB Wageningen, The Netherlands;

    Laboratory of Phytopathology, Wageningen University, 6708 PB Wageningen, The Netherlands;

    Graduate School of Horticulture, Chiba University, Matsudo, Chiba 271-8510, Japan;

    Scientia Terrae Research Institute, B-2860 Sint-Katelijne-Waver, Belgium,Laboratory for Process Microbial Ecology and Bioinspirational Management, Lessius University College, Campus De Nayer, Consortium for Industrial Microbiology Biotechnology, Department of Microbial and Molecular Systems, KU Leuyen Association, B-2860 Sint-Katelijne-Waver, Belgium;

    Department of Plant Pathology, University of California, Davis, CA 95616;

    Laboratory of Phytopathology, Wageningen University, 6708 PB Wageningen, The Netherlands,Centre for BioSystems Genomics, 6700 AB Wageningen, The Netherlands;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    fungus; genomics; elicitor; pathogen-associated molecular pattern;

    机译：菌;基因组学激发子病原体相关分子模式;

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