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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Robustness and accuracy of cell division in Escherichia coli in diverse cell shapes
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Robustness and accuracy of cell division in Escherichia coli in diverse cell shapes

机译：各种细胞形状下大肠杆菌细胞分裂的稳健性和准确性

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摘要

Cell division in typical rod-shaped bacteria such as Escherichia coli shows a remarkable plasticity in being able to adapt to a variety of irregular cell shapes. Here, we investigate the roles of the Min system and the nucleoid-occlusion factor SlmA in supporting this adaptation. We study "squeezed" E. coli in narrow nanofabricated channels where these bacteria exhibit highly-irregular shapes and large volumes. Despite the severely anomalous morphologies we find that most of these bacteria maintain their ability to divide into two equally sized daughters with an accuracy comparable to that of normal rod-shaped cells (about 4%). Deletion of either slmA or minC shows that the molecular systems associated with these genes are largely dispensable for accurate cell divfsion in these irregular cell shapes. Using fluorescence time-lapse microscopy, we determine that the functionality of the Min system is affected by the cell shape, whereas the localization of a nucleoid relative to the cell division proteins (the divisome) remains unperturbed in a broad spectrum of morphologies, consistent with nucleoid occlusion. The observed positioning of the nucleoid relative to the divisome appears not to be affected by the nucleoid-occlusion factor SlmA. The current study underscores the importance of nucleoid occlusion in positioning the divisome and shows that it is robust against shape irregularities.
机译：典型的棒状细菌（如大肠杆菌）中的细胞分裂显示出显着的可塑性，能够适应多种不规则的细胞形状。在这里，我们调查Min系统和类核苷酸闭塞因子SlmA在支持这种适应中的作用。我们在狭窄的纳米加工通道中研究“挤压”的大肠杆菌，在这些通道中，这些细菌表现出高度不规则的形状和大体积。尽管存在严重的异常形态，我们发现大多数细菌仍能以与正常杆状细胞（约4％）相当的精度将其分成两个大小相等的子代的能力。 slmA或minC的删除表明，与这些基因相关的分子系统对于在这些不规则细胞形状中进行精确的细胞分裂而言是非常必要的。使用荧光延时显微镜，我们确定Min系统的功能受细胞形状的影响，而相对于细胞分裂蛋白（小分子）的核苷定位在广泛的形态学中不受干扰，与核仁闭塞。所观察到的类核苷酸相对于小体的位置不受类核苷酸闭塞因子S1mA的影响。当前的研究强调了类核苷酸闭锁在定位小体中的重要性，并显示出它对形状不规则具有鲁棒性。

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  • 作者

    Jaan Mannik; Fabai Wu; Felix J. H. Hoi; Paola Bisicchia; David J. Sherratt; Juan E. Keyrner; Cees Dekker;

  • 作者单位

    Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The'Netherlands,Department of Physics and Astronomy, University of Tennessee, 1408 Circle Drive, Knoxville, TN 37996;

    Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The'Netherlands;

    Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The'Netherlands;

    Department of Biochemistry,University of Oxford, South Parks Road, Oxford 0X1 3QU, United Kingdom;

    Department of Biochemistry,University of Oxford, South Parks Road, Oxford 0X1 3QU, United Kingdom;

    Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The'Netherlands;

    Department of Bionanoscience, Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The'Netherlands;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cytokinesis; FtsZ; lab-on-a-chip; microfluidics;

    机译：胞质分裂FtsZ;芯片实验室微流体;

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