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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Membrane protein expression triggers chromosomal locus repositioning in bacteria
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Membrane protein expression triggers chromosomal locus repositioning in bacteria

机译：膜蛋白表达触发细菌中的染色体基因座重新定位

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摘要

It has long been hypothesized that subcellular positioning of chromosomal loci in bacteria may be influenced by gene function and expression state. Here we provide direct evidence that membrane protein expression affects the position of chromosomal loci in Escherichia coli. For two different membrane proteins, we observed a dramatic shift of their genetic loci toward the membrane upon induction. In related systems in which a cytoplasmic protein was produced, or translation was eliminated by mutating the start codon, a shift was not observed. Antibiotics that block transcription and translation similarly prevented locus repositioning toward the membrane. We also found that repositioning is relatively rapid and can be detected at positions that are a considerable distance on the chromosome from the gene encoding the membrane protein (>90 kb). Given that membrane protein-encoding genes are distributed throughout the chromosome, their expression may be an important mechanism for maintaining the bacterial chromosome in an expanded and dynamic state.
机译：长期以来一直认为，细菌中染色体基因座的亚细胞定位可能受基因功能和表达状态的影响。在这里，我们提供了直接的证据，表明膜蛋白表达影响大肠杆菌中染色体基因座的位置。对于两种不同的膜蛋白，我们观察到诱导后它们的遗传基因座向膜的急剧变化。在产生胞质蛋白或通过突变起始密码子消除翻译的相关系统中，未观察到移位。阻断转录和翻译的抗生素类似地阻止了基因座向膜的重新定位。我们还发现重新定位相对较快，并且可以在染色体上距编码膜蛋白的基因（> 90 kb）相当远的位置处检测到。考虑到膜蛋白编码基因分布在整个染色体中，它们的表达可能是维持细菌染色体处于扩展和动态状态的重要机制。

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