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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Reconstitution of abscisic acid activation of SLAC1 anion channel by CPK6 and OST1 kinases and branched ABU PP2C phosphatase action
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Reconstitution of abscisic acid activation of SLAC1 anion channel by CPK6 and OST1 kinases and branched ABU PP2C phosphatase action

机译：通过CPK6和OST1激酶和分支的ABU PP2C磷酸酶作用重建SLAC1阴离子通道的脱落酸活化

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摘要

The plant hormone abscisic acid (ABA) is produced in response to abiotic stresses and mediates stomatal closure in response to drought via recently identified ABA receptors (pyrabactin resistance/regulatory component of ABA receptor; PYR/RCAR). SLAC1 encodes a central guard cell S-type anion channel that mediates ABA-induced stomatal closure. Coexpression of the calcium-dependent protein kinase 21 (CPK21), CPK23, or the Open Stomata 1 kinase (OST1) activates SLAC1 anion currents. However, reconstitution of ABA activation of any plant ion channel has not yet been attained. Whether the known core ABA signaling components are sufficient for ABA activation of SLAC1 anion channels or whether additional components are required remains unknown. The Ca~(2+)-dependent protein kinase CPK6 is known to function in vivo in ABA-induced stomatal closure. Here we show that CPK6 robustly activates SLAC1 -mediated currents and phosphory-lates the SLAC1 N terminus. A phosphorylation site (S59) in SLAC1, crucial for CPK6 activation, was identified. The group A PP2Cs ABU, ABI2, and PP2CA down-regulated CPK6-mediated SLAC1 activity in oocytes. Unexpectedly, ABU directly dephosphorylated the N terminus of SLAC1, indicating an alternate branched early ABA signaling core in which ABU targets SLAC1 directly (down-regulation). Furthermore, here we have successfully reconstituted ABA-induced activation of SLAC1 channels in oocytes using the ABA receptor pyrabactin resistant 1 (PYR1) and PP2C phosphatases with two alternate signaling cores including either CPK6 or OST1. Point mutations in ABM disrupting PYR1-ABI1 interaction abolished ABA signal transduction. Moreover, by addition of CPK6, a functional ABA signal transduction core from ABA receptors to ion channel activation was reconstituted without a SnRK2 kinase.
机译：植物激素脱落酸（ABA）响应于非生物胁迫而产生，并通过最近鉴定出的ABA受体（对吡菌素的抗性/ ABA受体的调节成分； PYR / RCAR）响应干旱而介导气孔关闭。 SLAC1编码一个中央保卫细胞S型阴离子通道，该通道介导ABA诱导的气孔关闭。钙依赖性蛋白激酶21（CPK21），CPK23或开放气孔1激酶（OST1）的共表达激活SLAC1阴离子电流。但是，尚未实现任何植物离子通道的ABA活化重构。已知的核心ABA信号转导成分是否足以激活SLAC1阴离子通道的ABA或是否需要其他成分仍然未知。已知Ca（2+）依赖性蛋白激酶CPK6在ABA诱导的气孔关闭中在体内起作用。在这里，我们显示CPK6可以强有力地激活SLAC1介导的电流并磷酸化SLAC1 N末端。确定了SLAC1中对CPK6激活至关重要的磷酸化位点（S59）。 A组PP2Cs ABU，ABI2和PP2CA下调了卵母细胞中CPK6介导的SLAC1活性。出乎意料的是，ABU直接使SLAC1的N末端去磷酸化，表明ABU直接靶向SLAC1（下调）的另一个分支的早期ABA信号核心。此外，在这里，我们已经成功地重建了ABA诱导的卵母细胞中的SLAC1通道的激活，使用了ABA受体吡菌素抗性1（PYR1）和PP2C磷酸酶以及两个交替的信号核心，包括CPK6或OST1。破坏PYR1-ABI1相互作用的ABM中的点突变废除了ABA信号转导。此外，通过添加CPK6，从ABA受体到离子通道激活的功能性ABA信号转导核心得以重建，而无需SnRK2激酶。

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  • 作者

    Benjamin Brandt; Dennis E. Brodsky; Shaowu Xue; Juntaro Negi; Koh lba; Jaakko Kangasjaervi; Majid Ghassemian; Aaron B. Stephan; Honghong Hu; Julian I. Schroeder;

  • 作者单位

    Cell and Developmental Biology Section, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093;

    Cell and Developmental Biology Section, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093;

    Cell and Developmental Biology Section, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China;

    Department of Biology, Faculty of Sciences, Kyushu University, Fukuoka 812-8581, Japan;

    Department of Biology, Faculty of Sciences, Kyushu University, Fukuoka 812-8581, Japan;

    Division of Plant Biology, Department of Biosciences, University of Helsinki, FI-00014 Helsinki, Finland;

    Biomolecular/Proteomics Mass Spectrometry Facility, Department of Chemistry and Biochemistry, University of California at San Diego, La Jolla, CA 92093-0378;

    Cell and Developmental Biology Section, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093;

    Cell and Developmental Biology Section, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093;

    Cell and Developmental Biology Section, Division of Biological Sciences, University of California at San Diego, La Jolla, CA 92093;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    arabidopsis; chloride channel;

    机译：拟南芥;氯通道;

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