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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Thylakoid potassium channel is required for efficient photosynthesis in cyanobacteria
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Thylakoid potassium channel is required for efficient photosynthesis in cyanobacteria

机译：类固醇钾通道是蓝藻中有效光合作用所必需的

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摘要

A potassium channel (SynK) of the cyanobacterium Synechocystis sp. PCC 6803, a photoheterotrophic model organism for the study of photosynthesis, has been recently identified and demonstrated to function as a potassium selective channel when expressed in a heterologous system and to be located predominantly to the thylakoid membrane in cyanobacteria. To study its physiological role, a SynK-less knockout mutant was generated and characterized. Fluorimetric experiments indicated that SynK-less cyanobacteria cannot build up a proton gradient as efficiently as WT organisms, suggesting that SynK might be involved in the regulation of the electric component of the proton motive force. Accordingly, measurements of flash-induced cytochrome b6i turnover and respiration pointed to a reduced generation of ApH and to an altered linear electron transport in mutant cells. The lack of the channel did not cause an altered membrane organization, but decreased growth and modified the photosystem ll/photosystem I ratio at high light intensities because of enhanced photosensitiv-ity. These data shed light on the function of a prokaryotic potassium channel and reports evidence, by means of a genetic approach, on the requirement of a thylakoid ion channel for optimal photosynthesis.
机译：蓝藻Synechocystis sp。的钾通道（SynK）。 PCC 6803，一种用于光合作用研究的光异养模型生物，最近已被鉴定并证明在异源系统中表达时起钾选择性通道的作用，并且主要位于蓝细菌的类囊体膜中。为了研究其生理作用，生成并表征了无SynK的敲除突变体。荧光实验表明，无SynK的蓝细菌不能像WT生物那样有效地建立质子梯度，这表明SynK可能参与了质子动力电子成分的调节。因此，对闪光诱导的细胞色素b6i更新和呼吸的测量表明突变细胞中ApH的产生减少和线性电子传递的改变。通道的缺乏没有引起改变的膜组织，但是由于增强了光敏性，所以在高光强度下生长减少并且改变了光系统II /光系统I的比率。这些数据阐明了原核钾通道的功能，并通过遗传方法报告了类囊体离子通道对最佳光合作用的要求。

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  • 作者

    Vanessa Checchetto; Anna Segalla; Guillaume Allorent; Nicoletta La Rocca; Luigi Leanza; Giorgio Mario Giacometti; Nobuyuki Uozumi; Giovanni Finazzi; Elisabetta Bergantino; and lldiko Szabd;

  • 作者单位

    Department of Biology, University of Padova, 35121 Padova, Italy;

    Department of Biology, University of Padova, 35121 Padova, Italy;

    Centre National Recherche Scientifique, Unite Mixte Recherche (UMR) 5168, Laboratoire Physiologie Cellulaire et Vegetale, Commissariat a I'Energie Atomique et Energies Alternatives, F-38054 Grenoble, France Tlnstitut de Recherches en Technologies et Sciences pour le Vivant, Commissariat a I'Energie Atomique et Energies Alternatives, F-38054 Grenoble, France Universite Grenoble 1,F-38041, France lnstitut National Recherche Agronomique, UMR1200, F-38054 Grenoble, France;

    Department of Biology, University of Padova, 35121 Padova, Italy;

    Department of Biology, University of Padova, 35121 Padova, Italy;

    Department of Biology, University of Padova, 35121 Padova, Italy;

    Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan;

    Centre National Recherche Scientifique, Unite Mixte Recherche (UMR) 5168, Laboratoire Physiologie Cellulaire et Vegetale, Commissariat a I'Energie Atomique et Energies Alternatives, F-38054 Grenoble, France Tlnstitut de Recherches en Technologies et Sciences pour le Vivant, Commissariat a I'Energie Atomique et Energies Alternatives, F-38054 Grenoble, France Universite Grenoble 1,F-38041, France lnstitut National Recherche Agronomique, UMR1200, F-38054 Grenoble, France;

    Department of Biology, University of Padova, 35121 Padova, Italy;

    Department of Biology, University of Padova, 35121 Padova, Italy;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    bioenergetics; ion flux; membrane potential;

    机译：生物能学离子通量膜电位;

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