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The Wolfe cycle comes full circle

机译:沃尔夫周期到来

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In 1988, Rouviere and Wolfe (1) suggested that methane formation from H_2 and CO_2 by methanogenic archaea could be a cyclical process. Indirect evidence indicated that the first step, the reduction of CO_2 to for-mylmethanofuran, was somehow coupled to the last step, the reduction of the het-erodisulfide (CoM-S-S-CoB) to coenzyme M (CoM-SH) and coenzyme B (CoB-SH). Over 2 decades passed until the coupling mechanism was unraveled in 2011: Via flavin-based electron bifurcation, the reduction of CoM-S-S-CoB with H_2 provides the reduced ferredoxin (Fig. 1h) required for CO_2 reduction to formylmethanofuran (2) (Fig. la).
机译:1988年,Rouviere和Wolfe(1)提出,甲烷化古生菌从H_2和CO_2形成甲烷可能是一个循环过程。间接证据表明,第一步是将CO_2还原为甲代甲基呋喃,最后一步是将杂二硫化物(CoM-SS-CoB)还原为辅酶M(CoM-SH)和辅酶B。 (CoB-SH)。经过超过20年的时间,直到2011年揭示了偶联机理:通过基于黄素的电子分叉,用H_2还原CoM-SS-CoB提供了将CO_2还原为甲酰甲呋喃所需的还原铁氧还蛋白(图1h)(图2h)。 .la)。

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