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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Impairment of O-antigen production confers resistance to grazing in a model amoeba-cyanobacterium predator-prey system
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Impairment of O-antigen production confers resistance to grazing in a model amoeba-cyanobacterium predator-prey system

机译：在模型变形虫-蓝细菌捕食者-猎物系统中，O抗原产生的损害赋予了对放牧的抗性

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The grazing activity of predators on photosynthetic organisms is a major mechanism of mortality and population restructuring in natural environments. Grazing is also one of the primary difficulties in growing cyanobacteria and other microalgae in large, open ponds for the production of biof uels, as contaminants destroy valuable bio-mass and prevent stable, continuous production of biofuel crops. To address this problem, we have isolated a heterolobosean amoeba, HGG1, that grazes upon unicellular and filamentous freshwater cya-nobacterial species. We have established a model predator-prey system using this amoeba and Synechococcus elongatus PCC 7942. Application of amoebae to a library of mutants of 5. elongatus led to the identification of a grazer-resistant knockout mutant of the wzm ABC O-antigen transporter gene, SynPCC7942_1126. Mutations in three other genes involved in O-antigen synthesis and transport also prevented the expression of O-antigen and conferred resistance to HGG1. Complementation of these rough mutants returned O-antigen expression and susceptibility to amoebae. Rough mutants are easily identifiable by appearance, are capable of autoflocculation, and do not display growth defects under standard laboratory growth conditions, all of which are desired traits for a biofuel production strain. Thus, preventing the production of O-antigen is a pathway for producing resistance to grazing by certain amoebae.
机译：捕食者对光合生物的放牧活动是自然环境中死亡和种群重组的主要机制。放牧也是在大型开放池塘中生产蓝藻和其他微藻以生产生物燃料的主要困难之一，因为污染物会破坏有价值的生物量并阻止稳定，连续地生产生物燃料作物。为了解决这个问题，我们分离出了一种杂食性变形虫变形虫HGG1，它以单细胞和丝状淡水Cya-nobacterial物种为食。我们已经建立了使用该变形虫和细长突触球菌PCC 7942的捕食者-捕食者模型系统。将变形虫应用于5.细长突变体文库导致鉴定了wzm ABC O抗原转运蛋白基因的耐草食性敲除突变体，SynPCC7942_1126。参与O抗原合成和转运的其他三个基因的突变也阻止了O抗原的表达并赋予了对HGG1的抗性。这些粗糙突变体的补充使O-抗原表达和对变形虫易感。粗糙的突变体很容易通过外观识别，能够自絮凝，并且在标准实验室生长条件下不显示生长缺陷，所有这些都是生物燃料生产菌株所需的特性。因此，防止O-抗原的产生是产生对某些变形虫产生的抗性的途径。

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    Ryan Simkovsky; Emy F. Daniels; Karen Tang; Stacey C. Huynh; Susan S. Golden; Bianca Brahamsha;

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    Center for Chronobiology and Division of Biological Sciences Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093;

    Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093;

    Center for Chronobiology and Division of Biological Sciences Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093;

    Center for Chronobiology and Division of Biological Sciences Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093;

    Center for Chronobiology and Division of Biological Sciences Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093;

    Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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