...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Making male gametes in culture
【24h】

Making male gametes in culture

机译：在文化中制作雄配子

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Making sperm in a culture dish has been a long-term goal of reproductive biologists. In 2010, a group of investigators from Yokohama, Japan, reinvestigated an organ culture system that was originally used for testicular tissue in the 1960s (1-3). By using better molecular markers, they were able to demonstrate that some cells in this culture system underwent complete meiosis to form round sperma-tids (1). This work was followed by a study from the same research group that showed that the efficiency of the completion of meiosis and even the formation of flagellated cells was increased by the addition of knockout serum replacement to the organ culture medium (4). The investigators also showed that cryopreserved testicular tissue could be used in these cultures, and healthy, reproductively competent murine offspring were obtained after microinsemination using the haploid cells produced in culture. This technique
机译：在培养皿中制作精子一直是生殖生物学家的长期目标。 2010年，来自日本横滨的一组研究人员对器官培养系统进行了重新研究，该系统最初于1960年代用于睾丸组织（1-3）。通过使用更好的分子标记，他们能够证明该培养系统中的某些细胞经历了完全减数分裂，形成了圆形的精子。这项工作之后是来自同一研究小组的一项研究，该研究表明，通过向器官培养基中添加敲除血清替代品，可以提高减数分裂完成甚至鞭毛细胞形成的效率（4）。研究人员还表明，冷冻保存的睾丸组织可用于这些培养物中，并且使用培养物中产生的单倍体细胞进行微授精后，可获得健康，繁殖能力强的鼠后代。这项技术

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号