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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Assessment of morphometry of pulmonary acini in mouse lungs by nondestructive imaging using multiscale microcomputed tomography
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Assessment of morphometry of pulmonary acini in mouse lungs by nondestructive imaging using multiscale microcomputed tomography

机译：多尺度微计算机断层成像无损成像评估小鼠肺部肺腺泡形态

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摘要

Establishing the 3D architecture and morphometry of the intact pulmonary acinus is an essential step toward a more complete understanding of the relationship of lung structure and function. We combined a special fixation method with a unique volumetric nondestructive imaging technique and image processing tools to separate individual acini in the mouse lung. Interior scans of the parenchyma at a resolution of 2 urn enabled the reconstruction and quantitative study of whole acini by image analysis and ster-eologic methods, yielding data characterizing the 3D morphometry of the pulmonary acinus. The 3D reconstructions compared well with the architecture of silicon rubber casts of mouse acini. The image-based segmentation of individual acini allowed the computation of acinar volume and surface area, as well as estimation of the number of alveoli per acinus using stereologic methods. The acinar morphometry of male C57BL/6 mice age 12 wk and 91 wk was compared. Significant increases in all parameters as a function of age suggest a continuous change of the lung morphometry, with an increase in alveoli beyond what has been previously viewed as the maturation phase of the animals. Our image analysis methods open up opportunities for defining and quantitatively assessing the acinar structure in healthy and diseased lungs. The methods applied here to mice can be adjusted for the study of similarly prepared human lungs.
机译：建立完整的肺泡腺的3D体系结构和形态计量学是朝更完整地了解肺部结构与功能之间的关系迈出的重要一步。我们将特殊的固定方法与独特的体积无损成像技术和图像处理工具相结合，以分离小鼠肺中的单个腺泡。薄壁组织内部扫描的分辨率为2微米，可通过图像分析和立体生理学方法重建和定量研究整个腺泡，产生表征肺腺泡3D形态的数据。 3D重建与鼠标acini的硅橡胶铸件的体系结构进行了比较。对单个腺泡进行基于图像的分割，可以计算出腺泡的体积和表面积，并可以使用立体学方法估算每个腺泡的肺泡数量。比较了12周和91周的雄性C57BL / 6小鼠的腺泡形态。随着年龄的增长，所有参数的显着增加表明肺形态的不断变化，肺泡的增加超过了以前被视为动物成熟期的水平。我们的图像分析方法为定义和定量评估健康和患病肺中的腺泡结构提供了机会。可以调整此处应用于小鼠的方法，以研究相似制备的人肺。

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    Dragos. M. Vasilescu; Zhiyun Gao; Punam K. Saha; Leilei Yin; Ge Wang; Beatrice Haefeli-Bleuer; Matthias Ochs; Ewald R. Weibel; Eric A. Hoffman;

  • 作者单位

    Department of Radiology, University of Iowa, Iowa City, IA 52242,Department of Diagnostic Radiology, Philipps University, 35032 Marburg, Germany;

    Department of Radiology, University of Iowa, Iowa City, IA 52242,Department of Electrical Engineering, University of Iowa, Iowa City, IA 52242;

    Department of Radiology, University of Iowa, Iowa City, IA 52242,Department of Electrical Engineering, University of Iowa, Iowa City, IA 52242;

    Beckman Institute, University of Illinois, Urbana, IL 61801;

    Biomedical Engineering and Sciences, Virginia Tech, Blacksburg, VA 24061;

    Institute of Anatomy, University of Bern, Bern 9, Switzerland;

    Institute of Functional and Applied Anatomy, Hannover Medical School, 30625 Hannover, Germany;

    Institute of Anatomy, University of Bern, Bern 9, Switzerland;

    Department of Radiology, University of Iowa, Iowa City, IA 52242,Departments of Medicine Engineering, University of Iowa, Iowa City, IA 52242,Departments of Biomedical Engineering, University of Iowa, Iowa City, IA 52242;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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