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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Cellular complexity captured in durable silica biocomposites
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Cellular complexity captured in durable silica biocomposites

机译:耐用二氧化硅生物复合材料捕获的细胞复杂性

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摘要

Tissue-derived cultured cells exhibit a remarkable range of morphological features in vitro, depending on phenotypic expression and environmental interactions. Translation of these cellular architectures into inorganic materials would provide routes to generate hierarchical nanomaterials with stabilized structures and functions. Here, we describe the fabrication of cell/silica composites (CSCs) and their conversion to silica replicas using mammalian cells as scaffolds to direct complex structure formation. Under mildly acidic solution conditions silica deposition is restricted to the molecularly crowded cellular template. Inter- and intracellular heterogeneity from the nano- to macroscale is captured and dimensionally preserved in CSCs following drying and subjection to extreme temperatures allowing, for instance, size and shape preserving pyrolysis of cellular architectures to form conductive carbon replicas. The structural and behavioral malleability of the starting material (cultured cells) provides opportunities to develop robust and economical biocomposites with programmed structures and functions.
机译:组织衍生的培养细胞在体外表现出显着范围的形态学特征,这取决于表型表达和环境相互作用。这些细胞结构转化为无机材料将提供产生具有稳定结构和功能的分层纳米材料的途径。在这里,我们描述了细胞/二氧化硅复合材料(CSCs)的制造以及它们使用哺乳动物细胞作为支架来指导复杂结构形成的过程向二氧化硅复制品的转化。在弱酸性溶液条件下,二氧化硅沉积仅限于分子拥挤的细胞模板。在干燥和经受极端温度后,从纳米级到宏观级的细胞间和细胞内异质性被捕获并尺寸保存在CSC中,从而允许例如尺寸和形状保持细胞结构的热解以形成导电碳复制品。起始材料(培养细胞)的结构和行为可延展性提供了开发具有程序化结构和功能的健壮且经济的生物复合材料的机会。

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  • 作者单位

    Advanced Materials Laboratory, Sandia National Laboratories, albuquerque, NM 87106,Department of Chemical and Nuclear Engineering, University of New Mexico, albuquerque, NM 87131;

    Department of Chemical and Nuclear Engineering, University of New Mexico, albuquerque, NM 87131;

    Advanced Materials Laboratory, Sandia National Laboratories, albuquerque, NM 87106;

    Department of Chemical and Nuclear Engineering, University of New Mexico, albuquerque, NM 87131;

    Center for Integrated Nanotechnologies, albuquerque, NM 87185;

    Department of Chemical and Nuclear Engineering, University of New Mexico, albuquerque, NM 87131;

    Advanced Materials Laboratory, Sandia National Laboratories, albuquerque, NM 87106,Department of Chemical and Nuclear Engineering, University of New Mexico, albuquerque, NM 87131;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    sol-gel; biomineralization; biopreservation; frustule;

    机译:溶胶凝胶生物矿化;生物保存挫败;

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