...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Plasticity of the asialoglycoprotein receptor deciphered by ensemble FRET imaging and single-molecule counting PALM imaging
【24h】

Plasticity of the asialoglycoprotein receptor deciphered by ensemble FRET imaging and single-molecule counting PALM imaging

机译：整体FRET成像和单分子计数PALM成像破译的去唾液酸糖蛋白受体的可塑性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The plasma membrane contains numerous membrane receptors that assemble into complexes of differing complexity that influence their activity and ligand binding. Here, to better understand the relationships between receptor structure and function, we performed visualization studies on a prototypic two-subunit plasma membrane receptor, the asialoglycoprotein receptor, which binds and internalizes diverse classes of desialylated glycoproteins. The asialoglycoprotein receptor is crucial for removing thrombo-genic material (i.e., blood clotting factors and platelets) in sepsis or after transfusion of long-term stored platelets. Using ensemble FRET imaging and single-molecule counting photoactivated localization microscopy (PALM), we tested whether cells display different forms of the asialoglycoprotein receptor, what the subunit stoichiometries of these forms are, and whether they have distinct ligand specificities.
机译：质膜包含许多膜受体，这些膜受体组装成不同复杂性的复合物，影响其活性和配体结合。在这里，为了更好地理解受体结构和功能之间的关系，我们对原型的两个亚基质膜受体去唾液酸糖蛋白受体进行了可视化研究，该受体结合并内化了各种类型的去唾液酸化糖蛋白。去唾液酸糖蛋白受体对于在脓毒症或长期储存的血小板输注后去除血栓形成物质（即凝血因子和血小板）至关重要。使用整体FRET成像和单分子计数光活化定位显微镜（PALM），我们测试了细胞是否显示不同形式的去唾液酸糖蛋白受体，这些形式的亚基化学计量以及它们是否具有独特的配体特异性。

著录项

  • 来源
  • 作者

    Malte Renz; Brian R. Daniels; Gyoergy Vamosi; Irwin M. Arias; Jennifer Lippincott-Schwartz;

  • 作者单位

    Section on Organelle Biology The Eunice Kennedy Shriver National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, National Institutes of Health, Bethesda, MD 20892,Section on Organelle Biology Unit on Cellular Polarity, The Eunice Kennedy Shriver National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, National Institutes of Health, Bethesda, MD 20892;

    Section on Organelle Biology The Eunice Kennedy Shriver National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, National Institutes of Health, Bethesda, MD 20892;

    Department of Biophysics and Cell Biology, Medical and Health Science Center, University of Debrecen, H-4012 Debrecen, Hungary;

    Section on Organelle Biology Unit on Cellular Polarity, The Eunice Kennedy Shriver National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, National Institutes of Health, Bethesda, MD 20892;

    Section on Organelle Biology The Eunice Kennedy Shriver National Institute of Child Health and Human Development, Cell Biology and Metabolism Branch, National Institutes of Health, Bethesda, MD 20892;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号