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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Investigation of the sodium-binding sites in the sodium-coupled betaine transporter BetP
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Investigation of the sodium-binding sites in the sodium-coupled betaine transporter BetP

机译：钠偶联甜菜碱转运蛋白BetP中钠结合位点的研究

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摘要

The movement of small molecules across biological membranes against gradients of low-to-high concentration is facilitated by membrane-embedded transporter proteins: Many transporters obtain power for the process by coupling the process to the movement of ions down preexisting concentration gradients (Fig. PL4). Understanding the molecular mechanisms behind this coupling first requires detailed descriptions of the ion-binding sites within the transporters. Here, we identify the sodium-binding sites in a representative of a large superfamily of transporters that share a common structural fold. To do so, we used a combination of computational predictions and biochemical and biophysical analyses. The results led to the intriguing observation that the two sodium sites are located at positions within this fold that are related by the internal pseudosymmetry of the structure and provide clues as to how the sodium molds the protein structure to facilitate the transport cycle (Fig. P1B).
机译：膜嵌入的转运蛋白促进小分子在生物膜上针对低到高浓度梯度的运动：许多转运蛋白通过将过程与离子在预先存在的浓度梯度下的运动耦合来获得过程的动力（图PL4） ）。首先，了解这种偶联作用背后的分子机理需要对转运蛋白中离子结合位点的详细描述。在这里，我们确定了转运蛋白超家族的代表中的钠结合位点，它们具有共同的结构折叠。为此，我们将计算预测与生化和生物物理分析相结合。结果引起了一个有趣的观察，两个钠位点位于该折叠内的位置，这些位点与该结构的内部假对称性相关，并提供了有关钠如何塑造蛋白质结构以促进转运周期的线索（图P1B） ）。

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    Kamil Khafizov; Camilo Perez; Caroline Koshy; Matthias Quick; Klaus Fendler; Christine Ziegler; Lucy R. Forrest;

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    Computational Structural Biology Group and Departments of Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany,Department of Systems and Computational Biology, Albert Einstein College of Medicine, Bronx, NY 10461;

    Structural Biology Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany;

    Computational Structural Biology Group and Departments of Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany,Structural Biology Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany;

    Biophysical Chemistry, Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany,Center for Molecular Recognition Columbia University College of Physicians and Surgeons, New York, NY 10032;

    Department of Psychiatry, Columbia University College of Physicians and Surgeons, New York, NY 10032;

    Structural Biology Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany,Faculty of Biology and Preclinical Medicine, University of Regensburg, 93053 Regensburg, Germany;

    Computational Structural Biology Group and Departments of Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany,Faculty of Biology and Preclinical Medicine, University of Regensburg, 93053 Regensburg, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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