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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Structure of Sputnik, a virophage, at 3.5-A resolution
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Structure of Sputnik, a virophage, at 3.5-A resolution

机译：人造卫星Sputnik的结构，分辨率为3.5-A

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摘要

"Sputnik" is a dsDNA virus, referred to as a virophage, that is coassembied with Mimivirus in the host amoeba. We have used cryo-EM to produce an electron density map of the icosahedral Sputnik virus at 3.5-A resolution, sufficient to verify the identity of most amino acids in the capsid proteins and to establish the identity of the pentameric protein forming the fivefold vertices. It was also shown that the virus lacks an internal membrane. The capsid is organized into a T = 27 lattice in which there are 260 trimeric capsomers and 12 pentameric capsomers. The trimeric cap-somers consist of three double "jelly-roll" major capsid proteins creating pseudohexameric capsomer symmetry. The pentameric capsomers consist of five single jelly-roll proteins. The release of the genome by displacing one or more of the pentameric capsomers may be the result of a low-pH environment. These results suggest a mechanism of Sputnik DNA ejection that probably also occurs in other big icosahedral double jelly-roll viruses such as Adenovirus. In this study, the near-atomic resolution structure of a virus has been established where crystallization for X-ray crystallography was not feasible.
机译：“ Sputnik”是一种dsDNA病毒，称为病毒体，在宿主变形虫中与Mimivirus共同共存。我们已使用cryo-EM以3.5-A的分辨率产生二十面体Sputnik病毒的电子密度图，足以验证衣壳蛋白中大多数氨基酸的身份并确定形成五倍顶点的五聚体蛋白的身份。还显示该病毒缺乏内膜。衣壳组织成T = 27格，其中有260个三聚体和12个五聚体。三聚体异构体由三个双重“果冻卷”主要衣壳蛋白组成，形成假六聚体异构体对称性。五聚体的capsomers由五个单一的果冻卷蛋白组成。通过置换一种或多种五聚体的衣壳异构体而释放基因组可能是低pH环境的结果。这些结果表明Sputnik DNA弹出的机制可能也发生在其他大二十面体双胶冻卷病毒（如腺病毒）中。在这项研究中，已经建立了病毒的近原子分解结构，其中无法进行X射线晶体学结晶。

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  • 作者

    Xinzheng Zhang; Siyang Sun; Ye Xiang; Jimson Wong; Thomas Klose; Didier Raoult; Michael G. Rossmann;

  • 作者单位

    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907;

    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907;

    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907;

    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907;

    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907;

    Unite de Recherche sur les Maladies Infectieuses et Tropicales Emergentes, Unite Mixte de Recherche 6236 Centre National de la Recherche Scientifique, L'lnstitut de Recherche pour le Developpement 198, Faculte de Medecine, Universite de la Mediterranee, 13385 Marseille Cedex 5, France;

    Department of Biological Sciences, Purdue University, West Lafayette, IN 47907;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    dsDNA egress; virus assembly; mimivirus satellite; polypeptide tracing cryo-electron microscopy;

    机译：dsDNA出口;病毒组装;伪病毒卫星多肽示踪冷冻电子显微镜;

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