Intrinsic reaction-cycle time scale of Na~+,K~+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

Helene Bouvrais; Flemming Cornelius; John H. lpsen; Ole G. Mouritsen

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Intrinsic reaction-cycle time scale of Na~+,K~+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

【24h】

Intrinsic reaction-cycle time scale of Na~+,K~+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

机译：Na〜+，K〜+ -ATPase的内在反应周期时间尺度以非平衡膜的脂蛋白相互作用表现出来

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Interaction between integral membrane proteins and the lipid-bilayer component of biological membranes is expected to mutually influence the proteins and the membrane. We present quantitative evidence of a manifestation of the lipid-protein interactions in liposomal membranes, reconstituted with actively pumping Na~+,K~+-ATPase, in terms of nonequilibrium shape fluctuations that contain a relaxation time, t, which is robust and independent of the specific fluctuation modes of the membrane. In the case of pumping Nations, analysis of the flicker-noise temporal correlation spectrum of the liposomes leads to τ ≌ 0.5 s, comparing favorably with an intrinsic reaction-cycle time of about 0.4 s from enzymology.

机译：完整的膜蛋白和生物膜的脂双层成分之间的相互作用有望相互影响蛋白和膜。我们提供定量证据表明，脂质体膜中脂蛋白相互作用的表现是通过主动泵送Na〜+，K〜+ -ATPase进行重构的，这种非平衡形状波动包含松弛时间t，这是稳定且独立的膜的特定波动模式在泵浦民族的情况下，对脂质体的闪烁噪声时间相关光谱的分析导致τ≌0.5 s，与酶学中固有的约0.4 s的反应循环时间相比是有利的。

著录项

来源
《Proceedings of the National Academy of Sciences of the United States of America》 |2012年第45期|18442-18446|共5页
作者
Helene Bouvrais; Flemming Cornelius; John H. lpsen; Ole G. Mouritsen;
展开▼
作者单位

Department of Physics, Chemistry and Pharmacy, MEMPHYS-Center for Biomembrane Physics, University of Southern Denmark, 5230 Odense M, Denmark;

Department of Biomedicine, University of Aarhus, 8000 Aarhus C, Denmark;

Department of Physics, Chemistry and Pharmacy, MEMPHYS-Center for Biomembrane Physics, University of Southern Denmark, 5230 Odense M, Denmark;

Department of Physics, Chemistry and Pharmacy, MEMPHYS-Center for Biomembrane Physics, University of Southern Denmark, 5230 Odense M, Denmark;

展开▼
收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类
关键词
sodium pump reconstituted in giant vesicles; vesicle fluctuation analysis membrane dynamics;

机译：钠泵重组在大囊泡中;囊泡起伏分析膜动力学;

相似文献

外文文献
中文文献
专利

1. General and specific lipid-protein interactions in Na,K-ATPase [J] . Cornelius F., Habeck M., Kanai R., Biochimica et biophysica acta. Biomembranes . 2015,第9期

机译：Na，K-ATPase中一般和特定的脂蛋白相互作用
2. Lipid-protein interactions with the Na,K-ATPase [J] . Esmann M, Marsh D Chemistry and Physics of Lipids . 2006,第1a2期

机译：脂蛋白与Na，K-ATPase的相互作用
3. Selectivity of lipid-protein interactions with trypsinized Na,K-ATPase studied by spin-label EPR [J] . Ashish Arora, Mikael Esmann, Derek Marsh Biochimica et biophysica acta. Biomembranes . 1998,第2期

机译：旋转标记EPR研究脂蛋白与胰蛋白酶化的Na，K-ATPase相互作用的选择性
4. Lipid-protein interactions as determinants of membrane protein structure and function [C] . William Dowhan, Mikhail Bogdanov Biochemical Society Annual Symposium . 2011

机译：脂质蛋白质相互作用作为膜蛋白结构的决定因素和功能
5. BIOPHYSICAL CHARACTERIZATION OF LIPID-PROTEIN AND LIPID-LIPID INTERACTIONS IN MODEL AND RECONSTITUTED MEMBRANES USING STATIC AND REAL-TIME X-RAY DIFFRACTION AND FLUORESCENCE QUENCHING TECHNIQUES. [D] . CAFFREY, MARTIN DENIS. 1982

机译：使用静态和实时X射线衍射和荧光猝灭技术，对模型和重组膜中的脂蛋白和脂脂相互作用进行生物物理表征。
6. Intrinsic reaction-cycle time scale of Na+K+-ATPase manifests itself in the lipid–protein interactions of nonequilibrium membranes [O] . Hélène Bouvrais, Flemming Cornelius, John H. Ipsen, 2012

机译：Na +K + -ATPase的内在反应周期时标在非平衡膜的脂蛋白相互作用中表现出来
7. Bacterial osmosensing: roles of membrane structure and electrostatics in lipid-protein and protein-protein interactions:roles of membrane structure and electrostatics in lipid–protein and protein–protein interactions [O] . Poolman B., Spitzer J.J., Wood J.A., 2004

机译：细菌渗透：膜结构和静电在脂质-蛋白质和蛋白质-蛋白质相互作用中的作用：膜结构和静电在脂质-蛋白质和蛋白质-蛋白质相互作用中的作用
8. Resistance Model for Evaluating Interactions Between Natural Organic Matter (NOM) and Membranes at Different Scales of Operation. Desalination Research and Development Program Report, No. 44 [R] . Irvine, S., Amy, G. 1999

机译：不同尺度下评价天然有机物（NOm）与膜相互作用的抗性模型。海水淡化研究与发展计划报告，第44号

Intrinsic reaction-cycle time scale of Na~+,K~+-ATPase manifests itself in the lipid-protein interactions of nonequilibrium membranes

摘要

著录项

相似文献

相关主题

期刊订阅