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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Crystal structures of two subtype N10 neuraminidase-like proteins from bat influenza A viruses reveal a diverged putative active site
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Crystal structures of two subtype N10 neuraminidase-like proteins from bat influenza A viruses reveal a diverged putative active site

机译：甲型流感病毒的两个亚型N10神经氨酸酶样蛋白的晶体结构揭示了一个不同的假定活性位点

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摘要

Recently, we reported a unique influenza A virus subtype H17N10 from little yellow-shouldered bats. Its neuraminidase (NA) gene encodes a protein that appears to be highly divergent from all known influenza NAs and was assigned as a new subtype N10. To provide structural and functional insights on the bat H17N10 virus. X-ray structures were determined for N10 NA proteins from influenza A viruses A/little yellow-shouldered bat/Guatemala/164/ 2009 (GU09-164) in two crystal forms at 1.95 A and 2.5 A resolution and A/little yellow-shouldered bat/Guatemala/060/2010 (GU10-060) at 2.0 A. The overall N10 structures are similar to each other and to other known influenza NA structures, with a single highly conserved calcium binding site in each monomer. However, the region corresponding to the highly conserved active site of influenza A N1-N9 NA subtypes and influenza B NA differs substantially. In particular, most of the amino acid residues required for NA activity are substituted, and the putative active site is much wider because of displacement of the 150-loop and 430-loop. These structural features and the fact that the recombinant N10 protein exhibits no, or extremely low, NA activity suggest that it may have a different function than the NA proteins of other influenza viruses. Accordingly, we propose that the N10 protein be termed an NA-like protein until its function is elucidated.
机译：最近，我们报道了一种来自小黄色肩蝙蝠的独特甲型H17N10流感病毒。它的神经氨酸酶（NA）基因编码一种蛋白质，该蛋白质似乎与所有已知的流感NA高度不同，并被指定为新的N10亚型。提供有关蝙蝠H17N10病毒的结构和功能见解。确定了A流感病毒A /小黄肩蝙蝠/危地马拉/ 164/2009（GU09-164）的N10 NA蛋白的X射线结构，两种晶体形式的分辨率为1.95 A和2.5 A，A /小黄肩bat / Guatemala / 060/2010（GU10-060）在2.0 A下进行。总的N10结构彼此相似，与其他已知的流感NA结构相似，每个单体中都有一个高度保守的钙结合位点。然而，对应于甲型流感N1-N9NA亚型和乙型流感NA的高度保守的活性位点的区域存在很大差异。特别地，NA活性所需的大多数氨基酸残基被取代，并且由于150环和430环的置换，推定的活性位点宽得多。这些结构特征和重组N10蛋白不显示或不显示极低的NA活性这一事实表明，它可能具有与其他流感病毒的NA蛋白不同的功能。因此，我们建议直到阐明其功能之前，将N10蛋白称为NA样蛋白。

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  • 作者

    Xueyong Zhu; Hua Yang; Zhu Guo; Wenli Yu; Paul J. Carney; Yan Li; Li-Mei Chen; James C. Paulson; Ruben O. Donis; Suxiang Tong; James Stevens; Ian A. Wilson;

  • 作者单位

    Departments of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037;

    Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30333;

    Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30333;

    Departments of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037;

    Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30333;

    Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333;

    Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30333;

    Departments of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037,Departments of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037;

    Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30333;

    Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333;

    Influenza Division, Centers for Disease Control and Prevention, Atlanta, GA 30333;

    Departments of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037,Departments of Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    enzyme mechanism; receptor; glycoprotein; infection;

    机译：酶机制受体糖蛋白感染;

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