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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Quantitative single-cell characterization of bacterial interactions reveals type VI secretion is a double-edged sword
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Quantitative single-cell characterization of bacterial interactions reveals type VI secretion is a double-edged sword

机译:细菌相互作用的定量单细胞表征揭示了VI型分泌是一把双刃剑

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摘要

Interbacterial interaction pathways play an important role in defining the structure and complexity of bacterial associations. A quantitative description of such pathways offers promise for understanding the forces that contribute to community composition. We developed time-lapse fluorescence microscopy methods for quantitation of interbacterial interactions and applied these to the characterization of type VI secretion (T6S) in Pseudomonas aerugi-nosa. Our analyses allowed a direct determination of the efficiency of recipient cell lysis catalyzed by this intercellular toxin delivery pathway and provided evidence that its arsenal extends beyond known effector proteins. Measurement of T6S apparatus localization revealed correlated activation among neighboring cells, which, taken together with genetic data, implicate the elaboration of a functional T6S apparatus with a marked increase in susceptibility to intoxication. This possibility was supported by the identification of T6S-inactivating mutations in a genome-wide screen for resistance to T6S-mediated intoxication and by time-lapse fluorescence microscopy analyses showing a decreased lysis rate of recipient cells lacking T6S function. Our discoveries highlight the utility of single-cell approaches for measuring interbacterial phenomena and provide a foundation for studying the contribution of a widespread bacterial interaction pathway to community structure.
机译:细菌间的相互作用途径在定义细菌缔合的结构和复杂性方面起着重要作用。对这些途径的定量描述为了解有助于社区组成的力量提供了希望。我们开发了用于定量细菌间相互作用的延时荧光显微镜方法,并将其应用于铜绿假单胞菌VI型分泌(T6S)的表征。我们的分析可以直接确定由这种细胞间毒素传递途径催化的受体细胞裂解的效率,并提供证据表明其砷库已超出已知的效应蛋白。对T6S装置定位的测量表明,相邻细胞之间存在相关的激活,这与遗传数据一起暗示了对功能性T6S装置的精心设计,使其对中毒的敏感性显着增加。在全基因组筛选中对T6S介导的中毒具有抗性,并通过延时荧光显微镜分析表明缺乏T6S功能的受体细胞裂解率降低,从而证实了这种可能性。我们的发现突出了单细胞方法在测量细菌间现象方面的实用性,并为研究广泛的细菌相互作用途径对群落结构的贡献提供了基础。

著录项

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  • 作者单位

    Departments of Microbiology University of Washington, Seattle, WA 98195,Molecular and Cellular Biology Program, University of Washington, Seattle WA 98195;

    Departments of Microbiology University of Washington, Seattle, WA 98195;

    Departments of Physics, and University of Washington, Seattle, WA 98195,Departments of Bioengineering, University of Washington, Seattle, WA 98195;

    Departments of Microbiology University of Washington, Seattle, WA 98195;

    Departments of Microbiology University of Washington, Seattle, WA 98195;

    Departments of Microbiology University of Washington, Seattle, WA 98195;

    Departments of Microbiology University of Washington, Seattle, WA 98195;

    Departments of Physics, and University of Washington, Seattle, WA 98195;

    Departments of Physics, and University of Washington, Seattle, WA 98195,Departments of Bioengineering, University of Washington, Seattle, WA 98195;

    Departments of Microbiology University of Washington, Seattle, WA 98195;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    imaging; peptidoglycan; polymicrobial; protein secretion;

    机译:成像肽聚糖微生物蛋白质分泌;

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