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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Injectable preformed scaffolds with shape-memory properties
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Injectable preformed scaffolds with shape-memory properties

机译:具有形状记忆特性的可注射预制支架

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摘要

Injectable biomaterials are increasingly being explored to minimize risks and complications associated with surgical implantation. We describe a strategy for delivery via conventional needle-syringe injection of large preformed macroporous scaffolds with well-defined properties. Injectable 3D scaffolds, in the form of elastic sponge-like matrices, were prepared by environmentally friendly cryotropic gelation of a naturally sourced polymer. Cryogels with shape-memory properties may be molded to a variety of shapes and sizes, and may be optionally loaded with therapeutic agents or cells. These scaffolds have the capability to withstand reversible deformations at over 90% strain level, and a rapid volumetric recovery allows the structurally defined scaffolds to be injected through a small-bore needle with nearly complete geometric restoration once delivered. These gels demonstrated long-term release of biomolecules in vivo. Furthermore, cryogels impregnated with bioluminescent reporter cells provided enhanced survival, higher local retention, and extended engraftment of transplanted cells at the injection site compared with a standard injection technique. These injectable scaffolds show great promise for various biomedical applications, including cell therapies.
机译:越来越多地探索可注射生物材料以最小化与手术植入相关的风险和并发症。我们描述了一种通过常规的针头注射方式对具有明确定义的性质的大型预制大孔支架进行递送的策略。弹性海绵状基质形式的可注射3D支架是通过对天然来源的聚合物进行环保的低温凝胶化而制备的。具有形状记忆特性的冰晶可以模制成各种形状和尺寸,并且可以任选地装载治疗剂或细胞。这些支架具有承受超过90%应变水平的可逆变形的能力,并且快速的体积恢复能力使结构确定的支架通过小口径针头注入,一旦交付,其几何形状几乎完全恢复。这些凝胶证明了生物分子在体内的长期释放。此外,与标准注射技术相比,浸渍有生物发光报告细胞的冷冻凝胶可提高存活率,提高局部保留率并延长移植部位在移植部位的移植。这些可注射支架在各种生物医学应用(包括细胞疗法)中显示出巨大的希望。

著录项

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  • 作者单位

    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138,Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115;

    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138;

    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138;

    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138,Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115;

    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138;

    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138,Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115;

    School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138,Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA 02115;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    syringe-injectable hydrogels; cryogelation; shape retention; controlled delivery; cell therapy;

    机译:注射器可注射水凝胶;冷冻凝胶化形状保持力;控制交付;细胞疗法;

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