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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Direct visualization of the alamethicin pore formed in a planar phospholipid matrix
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Direct visualization of the alamethicin pore formed in a planar phospholipid matrix

机译：直接可视化在平面磷脂基质中形成的alamethicin孔

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摘要

We present direct visualization of pores formed by alamethicin (Alm) in a matrix of phospholipids using electrochemical scanning tunneling microscopy (EC-STM). High-resolution EC-STM images show individual peptide molecules forming channels. The channels are not dispersed randomly in the monolayer but agglomerate forming 2D nanocrystals with a hexagonal lattice in which the average channel-channel distance is 1.90 ± 0.1 nm. The STM images suggest that each Alm is shared between the two adjacent channels. Every channel consists of six Alm molecules. Three or four of these molecules have the hydrophilic group oriented toward the center of the channel allowing for water column formation inside the channel. The dimensions of the central pore in the images are consistent with the dimension of the water column in a model of hexameric pore proposed in the literature. The images obtained in this work validate the barrel-stave model of the pore formed in phospholipid membranes by amphiphatic pep-tides. They also provide direct evidence for cluster formation by such pores.
机译：我们目前使用电化学扫描隧道显微镜（EC-STM）在磷脂基质中由缩水甘油酯（Alm）形成的孔的直接可视化。高分辨率EC-STM图像显示单个肽分子形成通道。通道不是随机分散在单层中，而是团聚形成具有六方晶格的2D纳米晶体，其中平均通道-通道距离为1.90±0.1 nm。 STM图像表明每个Alm在两个相邻通道之间共享。每个通道由六个Alm分子组成。这些分子中的三个或四个具有朝向通道中心定向的亲水基团，从而允许在通道内形成水柱。图像中中心孔的尺寸与文献中提出的六聚体孔模型中水柱的尺寸一致。在这项工作中获得的图像验证了由两亲肽在磷脂膜上形成的孔的桶-梯级模型。它们还提供了通过这些孔形成簇的直接证据。

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  • 作者

    Piotr Pieta; Jeff Mirza; Jacek Lipkowski;

  • 作者单位

    Department of Chemistry, University of Guelph, Guelph, ON, Canada N1G 2W1,Department of Physical Chemistry of Supramolecular Complexes,Institute of Physical Chemistry, Polish Academy of Sciences, 01-224 Warsaw, Poland;

    Department of Chemistry, University of Guelph, Guelph, ON, Canada N1G 2W1;

    Department of Chemistry, University of Guelph, Guelph, ON, Canada N1G 2W1;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    antimicrobial; interface; langmuir-blodgett;

    机译：抗菌剂接口;朗缪尔-布洛杰特;

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