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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation
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Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation

机译：能够同时进行纤维二糖和木糖发酵的工程酿酒酵母

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摘要

The use of plant biomass for biofuel production will require efficient utilization of the sugars in lignocellulose, primarily glucose and xylose. However, strains of Saccharomyces cerevisiae presently used in bioethanol production ferment glucose but not xylose. Yeasts engineered to ferment xylose do so slowly, and cannot utilize xylose until glucose is completely consumed. To overcome these bottlenecks, we engineered yeasts to coferment mixtures of xylose and cellobiose. In these yeast strains, hydrolysis of cellobiose takes place inside yeast cells through the action of an intracellular β-glucosidase following import by a high-affinity cellodextrin transporter. Intracellular hydrolysis of cellobiose minimizes glucose repression of xylose fermentation allowing cocon-sumption of cellobiose and xylose. The resulting yeast strains, cofermented cellobiose and xylose simultaneously and exhibited improved ethanol yield when compared to fermentation with either cellobiose or xylose as sole carbon sources. We also observed improved yields and productivities from cofermentation experiments performed with simulated cellulosic hydrolyzates, suggesting this is a promising cofermentation strategy for cellulosic biofuel production. The successful integration of cellobiose and xylose fermentation pathways in yeast is a critical step towards enabling economic biofuel production.
机译：将植物生物质用于生物燃料生产将需要有效利用木质纤维素中的糖，主要是葡萄糖和木糖。然而，目前在生物乙醇生产中使用的酿酒酵母菌株发酵葡萄糖而不发酵木糖。经过改造的发酵木糖的酵母发酵过程缓慢，在葡萄糖被完全消耗之前无法利用木糖。为了克服这些瓶颈，我们设计了酵母以发酵木糖和纤维二糖的混合物。在这些酵母菌株中，纤维二糖的水解在细胞内β-葡萄糖苷酶的作用下通过高亲和力纤维糊精转运蛋白的导入而在酵母细胞内部发生。纤维二糖的细胞内水解可最大程度地减少木糖发酵的葡萄糖抑制，从而可共同消耗纤维二糖和木糖。与使用纤维二糖或木糖作为唯一碳源的发酵相比，所得的酵母菌株，纤维二糖和木糖同时发酵，并显示出更高的乙醇收率。我们还从模拟纤维素水解产物进行的发酵实验中观察到了提高的产量和生产率，这表明这是纤维素生物燃料生产的有希望的发酵策略。纤维二糖和木糖发酵途径在酵母中的成功整合是实现经济生物燃料生产的关键步骤。

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  • 作者

    Suk-Jin Ha; Jonathan M. Galazka; Soo Rin Kim; Jin-Ho Choi; Xiaomin Yang; Jin-Ho Seo; N. Louise Glass; Jamie H. D. Cate; Yong-Su Jin;

  • 作者单位

    Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801,Institute for Genomic Biology, University of Illinois, Urbana,IL 61801,Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720;

    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720;

    Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801,Institute for Genomic Biology, University of Illinois, Urbana,IL 61801;

    Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801,Institute for Genomic Biology, University of Illinois, Urbana,IL 61801;

    BP Biofuels Business Unit, Berkeley, CA 97420;

    Department of Agricultural Biotechnology, Seoul National University, Seoul 152-742, Korea;

    Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720;

    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720,Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720;

    Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801,Institute for Genomic Biology, University of Illinois, Urbana,IL 61801;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    biofuels; cellodextrin transporter; cofermentation; intracellular β-glucosidase;

    机译：生物燃料纤维糊精转运蛋白;合作细胞内β-葡萄糖苷酶;

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