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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6
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Execution of the meiotic noncoding RNA expression program and the onset of gametogenesis in yeast require the conserved exosome subunit Rrp6

机译：减数分裂非编码RNA表达程序的执行和酵母中配子发生的开始需要保守的外泌体亚基Rrp6

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摘要

Budding yeast noncoding RNAs (ncRNAs) are pervasively transcribed during mitosis, and some regulate mitotic protein-coding genes. However, little is known about ncRNA expression during meiotic development. Using high-resolution profiling we identified an extensive meiotic ncRNA expression program interlaced with the protein-coding transcriptome via sense/antisense transcript pairs, bidirectional promoters, and ncRNAs that overlap the regulatory regions of genes. Meiotic unannotated transcripts (MUTs) are mitotic targets of the conserved exosome component Rrp6, which itself is degraded after the onset of meiosis when MUTs and other ncRNAs accumulate in successive waves. Diploid cells lacking Rrp6 fail to initiate premeiotic DNA replication normally and cannot undergo efficient meiotic development. The present study demonstrates a unique function for budding yeast Rrp6 in degrading distinct classes of meiotically induced ncRNAs during vegetative growth and the onset of meiosis and thus points to a critical role of differential ncRNA expression in the execution of a conserved developmental program.
机译：芽殖酵母非编码RNA（ncRNA）在有丝分裂过程中普遍转录，并且某些调控有丝分裂蛋白编码基因。但是，关于减数分裂发育过程中ncRNA表达的了解甚少。使用高分辨率分析，我们确定了一个广泛的减数分裂ncRNA表达程序，该程序通过有义/反义转录物对，双向启动子和与基因调控区重叠的ncRNA与蛋白质编码转录组交错排列。减数分裂未注释的转录本（MUT）是保守的外泌体成分Rrp6的有丝分裂靶标，当减数分裂发作时MUT和其他ncRNA在连续波中累积时，其自身会降解。缺乏Rrp6的二倍体细胞无法正常启动减数分裂前DNA复制，并且无法进行有效的减数分裂发育。本研究表明，在营养生长和减数分裂发生过程中，酵母Rrp6在降解不同种类的减数分裂诱导的ncRNA中具有独特的功能，因此指出了差异ncRNA表达在执行保守的发育程序中的关键作用。

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  • 作者

    Aurelie Lardenois; Yuchen Liu; Thomas Walther; Frederic Chalmel; Bertrand Evrard; Marina Granovskaia; Angela Chu; Ronald W. Davis; Lars M. Steinmetz; Michael Primig;

  • 作者单位

    Institut National de la Sante et de la Recherche Medicale, Unite 625, Institut Federatif de Recherche 140, Universite de Rennes 1, F-35042 Rennes, France;

    Institut National de la Sante et de la Recherche Medicale, Unite 625, Institut Federatif de Recherche 140, Universite de Rennes 1, F-35042 Rennes, France;

    Unite Mixte de Recherche (UMR) Institut National des Sciences Appliquees (INSA)/Centre National de la Recherche Scientifique 5504 and UMR INSA/lnstitutNational de la Recherche Agronomique 792, F-31077 Toulouse, France;

    Institut National de la Sante et de la Recherche Medicale, Unite 625, Institut Federatif de Recherche 140, Universite de Rennes 1, F-35042 Rennes, France;

    Institut National de la Sante et de la Recherche Medicale, Unite 625, Institut Federatif de Recherche 140, Universite de Rennes 1, F-35042 Rennes, France;

    European Molecular Biology Laboratory, 69117 Heidelberg, Germany;

    Stanford Genome Technology Center, Palo Alto, CA 94304;

    Stanford Genome Technology Center, Palo Alto, CA 94304,Department of Biochemistry, Stanford University, Stanford, CA 94305;

    European Molecular Biology Laboratory, 69117 Heidelberg, Germany;

    Institut National de la Sante et de la Recherche Medicale, Unite 625, Institut Federatif de Recherche 140, Universite de Rennes 1, F-35042 Rennes, France;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    saccharomyces cerevisiae; sporulation; tiling arrays;

    机译：酿酒酵母;孢子形成;平铺;

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