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Extranuclear protection of chromosomal DNA from oxidative stress

机译:核DNA免受氧化应激对染色体DNA的保护

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摘要

Eukaryotic organisms evolved under aerobic conditions subjecting nuclear DNA to damage provoked by reactive oxygen species (ROS). Although ROS are thought to be a major cause of DNA damage, little is known about the molecular mechanisms protecting nuclear DNA from oxidative stress. Here we show that protection of nuclear DNA in plants requires a coordinated function of ROS-scavenging pathways residing in the cytosol and perox-isomes, demonstrating that nuclear ROS scavengers such as peroxiredoxin and glutathione are insufficient to safeguard DNA integrity. Both catalase (CAT2) and cytosolic ascorbate peroxidase (APX1) play a key role in protecting the plant genome against photorespiratory-dependent H_2O_2-induced DNA damage. In apx1/ cat2 double-mutant plants, a DNA damage response is activated, suppressing growth via a WEE1 kinase-dependent cell-cycle checkpoint. This response is correlated with enhanced tolerance to oxidative stress, DNA stress-causing agents, and inhibited programmed cell death.
机译:真核生物在有氧条件下进化,使核DNA受到活性氧(ROS)的破坏。尽管人们认为ROS是造成DNA损伤的主要原因,但是对于保护核DNA免受氧化应激的分子机制知之甚少。在这里,我们表明保护植物中的核DNA需要协调清除细胞质和过氧化物酶体中的ROS清除途径的功能,这表明核ROS清除剂(如过氧化物酶和谷胱甘肽)不足以保护DNA完整性。过氧化氢酶(CAT2)和胞质抗坏血酸过氧化物酶(APX1)在保护植物基因组免受光呼吸依赖性H_2O_2诱导的DNA损伤中起关键作用。在apx1 / cat2双突变植物中,DNA损伤反应被激活,通过WEE1激酶依赖性细胞周期检查点抑制生长。这种反应与对氧化应激,DNA应激引起剂的耐受性增强和抑制的程序性细胞死亡相关。

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  • 作者单位

    Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium,Department of Plant Biotechnology and Genetics, Ghent University, 9052 Ghent, Belgium;

    Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557;

    Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557;

    Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium,Department of Plant Biotechnology and Genetics, Ghent University, 9052 Ghent, Belgium;

    Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium,Department of Plant Biotechnology and Genetics, Ghent University, 9052 Ghent, Belgium;

    Commissariat a I'Energie Atomique, Laboratoire des Lesions des Acides Nucleiques, Institut Nanosciences et Cryogenie, 38054 Grenoble Cedex 9, France,Universite Joseph Fourier, Centre National de la Recherche Scientifique, Laboratoire de Chimie Inorganique et Biologique (Unite Mixte de Recherche E 3-Commissariat a I'Energie Atomique-Universite Joseph Fourier- Formation de Recherche en Evolution 3200), 38041 Grenoble Cedex 9, France;

    Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557;

    Commissariat a I'Energie Atomique, Direction des Sciences du Vivant, Institut de Biologie Environnementale et de Biotechnologie, Laboratoire d'Ecophysiologie Moleculaire des Plantes, 13108 Saint-Paul-lez-Durance, France,Centre National de la Recherche Scientifique, Unite Mixte de Recherche, Biologie Vegetale et Microbiologie Environnementales, Univarsite d'Aix Marseille, 13108 Saint-Paul-lez-Durance, France;

    Department of Horticulture, Virginia Bioinformatics Institute, Blacksburg, VA 24061,Department of Biological Sciences, University of North Texas, Denton, TX 76203;

    Department of Plant Biotechnology and Genetics, Ghent University, 9052 Ghent, Belgium;

    Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium,Department of Plant Biotechnology and Genetics, Ghent University, 9052 Ghent, Belgium;

    Department of Biological Sciences, University of North Texas, Denton, TX 76203,Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    arabidopsis; stress tolerance; hydrogen peroxide;

    机译:拟南芥;压力容忍;过氧化氢;

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