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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >mut-16 and other mutator class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans
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mut-16 and other mutator class genes modulate 22G and 26G siRNA pathways in Caenorhabditis elegans

机译：mut-16和其他突变子基因调控秀丽隐杆线虫中的22G和26G siRNA途径

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摘要

Argonaute-associated siRNAs and Piwi-associated piRNAs have overlapping roles in silencing mobile genetic elements in animals. In Caenorhabditis elegans, mutator (mut) class genes mediate siRNA-guided repression of transposons as well as exogenous RNAi, but their roles in endogenous RNA silencing pathways are hot well-understood. To characterize the endogenous small RNAs dependent on mut class genes, small RNA populations from a null allele of mut-16 as well as a regulatory mut-16(mg461) allele that disables only somatic RNAi were subjected to deep sequencing. Additionally, each of the mut class genes was tested for a requirement in 26G siRNA pathways. The results indicate that mut-16 is an essential factor in multiple endogenous germline and somatic siRNA pathways involving several distinct Argonautes and RNA-dependent RNA polymerases. The results also reveal essential roles for mut-2 and mut-7 in the ERGO-1 class 26G siRNA pathway and less critical roles for mut-8, mut-14, and mut-15. We show that transposons are hypersusceptible to mut-76-dependent silencing and identify a requirement for the siRNA machinery in piRNA biogenesis from Tc1 transposons. We also show that the soma-specific mut-16(mg461) mutant allele is present in multiple C. elegans laboratory strains.
机译：Argonaute相关的siRNA和Piwi相关的piRNA在沉默动物的移动遗传元件方面具有重叠的作用。在秀丽隐杆线虫中，突变（mut）类基因介导siRNA指导的转座子以及外源RNAi的阻遏，但它们在内源RNA沉默途径中的作用已广为人知。为了表征依赖于mut类基因的内源性小RNA，对来自mut-16无效等位基因以及仅使体RNAi失能的调节性mut-16（mg461）等位基因的小RNA群体进行了深度测序。另外，测试了每个mut类基因对26G siRNA途径的需求。结果表明，mut-16是涉及多个不同的Argonautes和RNA依赖性RNA聚合酶的多种内源种系和体细胞siRNA途径中的重要因素。结果还揭示了mut-2和mut-7在ERGO-1类26G siRNA途径中的重要作用，而对于mut-8，mut-14和mut-15的作用则不太重要。我们显示转座子对mut-76依赖的沉默是高度敏感的，并确定了从Tc1转座子的piRNA生物发生中siRNA机制的需求。我们还显示，在多个秀丽隐杆线虫实验室菌株中存在特定于体细胞的mut-16（mg461）突变体等位基因。

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    Chi Zhang; Taiowa A. Montgomery; Harrison W. Gabel; Sylvia E. J. Fischer; Carolyn M. Phillips; Noah Fahlgren; Christopher M. Sullivan; James C. Carrington; Gary Ruvkun;

  • 作者单位

    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114,Department of Neurobiology, Harvard Medical School, Boston, MA 021 15;

    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114,Department of Neurobiology, Harvard Medical School, Boston, MA 021 15;

    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114,Department of Neurobiology, Harvard Medical School, Boston, MA 021 15;

    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114;

    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114;

    Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR 97331;

    Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR 97331;

    Center for Genome Research and Biocomputing, Oregon State University, Corvallis, OR 97331;

    Department of Molecular Biology, Massachusetts General Hospital, Department of Genetics, Harvard Medical School, Boston, MA 02114;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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