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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Atomic model of a cypovirus built from cryo-EM structure provides insight into the mechanism of mRNA capping
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Atomic model of a cypovirus built from cryo-EM structure provides insight into the mechanism of mRNA capping

机译：由cryo-EM结构构建的杯状病毒的原子模型提供了对mRNA封端机制的深入了解

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摘要

The cytoplasmic polyhedrosis virus (CPV) from the family Reovlri-dae belongs to a subgroup of "turreted" reoviruses, in which the mRNA capping activity occurs in a pentameric turret. We report a full atomic model of CPV built from a 3D density map obtained using cryoelectron microscopy. The image data for the 3D reconstruction were acquired exclusively from a CCD camera. Our structure shows that the enzymatic domains of the pentameric turret of CPV are topologically conserved and that there are five unique channels connecting the guanylyltransferase and methyltransferase regions. This structural organization reveals how the channels guide nascent mRNA sequentially to guanylyltransferase, 7-N-methyltransferase, and 2'-O-methyltransferase in the turret, undergoing the highly coordinated mRNA capping activity. fuerthermore, by fitting the deduced amino acid sequence of the protein VP5 to 120 large protrusion proteins on the CPV capsid shell, we confirmed that this protrusion protein is encoded by CPV RNA segment 7.
机译：来自Reovlri-dae家族的胞质多角体病毒（CPV）属于“转塔型”呼肠孤病毒的一个亚组，其中mRNA封盖活性发生在五聚体转塔中。我们报告了一个完整的CPV原子模型，该模型由使用低温电子显微镜获得的3D密度图构建而成。用于3D重建的图像数据仅从CCD摄像机获取。我们的结构表明，CPV五聚体转塔的酶结构域在拓扑上是保守的，并且存在五个连接胍基转移酶和甲基转移酶区域的独特通道。这种结构组织揭示了通道如何将新生的mRNA依次引导至转塔中的鸟苷酸转移酶，7-N-甲基转移酶和2'-O-甲基转移酶，并经历高度协调的mRNA封盖活性。此外，通过将推定的蛋白质VP5氨基酸序列与CPV衣壳上的120个大突出蛋白相匹配，我们确认该突出蛋白由CPV RNA片段7编码。

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    Lingpeng Cheng; Jingchen Sun; Kai Zhang; Zongjun Mou; Xiaoxing Huang; Gang Ji; Fei Sun; Jingqiang Zhang; Ping Zhu;

  • 作者单位

    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China;

    Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China;

    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China;

    Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, 483 Wushan Road, Guangzhou 510642, China;

    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China;

    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China;

    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China;

    State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, 135 Xingangxi Road, Guangzhou 510275, China;

    National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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