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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Embryonic day 9 yolk sac and intra-embryonic hemogenic endothelium independently generate a B-1 and marginal zone progenitor lacking B-2 potential
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Embryonic day 9 yolk sac and intra-embryonic hemogenic endothelium independently generate a B-1 and marginal zone progenitor lacking B-2 potential

机译：胚胎第9天卵黄囊和胚胎内造血内皮细胞独立产生B-1和缺乏B-2电位的边缘区祖细胞

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摘要

The majority of B lymphocytes in the adult mouse are generated in the bone marrow from hematopoietic stem cells (HSCs) that first appear in the aorta-gonado-mesonephros region of the fetus on embryonic day (E) 10.5-11. Comparatively less is known about B-cell development during embryogenesis. For example, which specific embryonic tissues participate in B lymphopoiesis and whether hematopoietic differentiation is skewed toward specific B-cell subsets in the embryo are unanswered questions, because the systemic circulation is initiated early during embryogenesis, resulting in an admixture of cells potentially originating from multiple sites. We demonstrate, using Ncx1~(-/-) mice that lack systemic blood circulation, that the E9 yolk sac (YS) and the intra-embryonic para-aortic splanch-nopleura (P-Sp) tissues independently give rise to AA4.1*CD19+ B220~(io-neg) B progenitor cells that preferentially differentiate into innate type B-1 and marginal zone (MZ) B cells but not into B-2 cells upon transplantation. We have fuerther demonstrated that these B-1 progenitor cells arise directly from YS and P-Sp hemogenic endothe-lium. These results document the initial wave of innate B lymphopoietic progenitor cells available for seeding the fetal liver at E11. The results of these studies expand our knowledge of hemogenic endo-thelial sites specifying distinct B-1 and MZ cell fates apart from B-2 cells and independent of an HSC origin during development.
机译：成年小鼠中的大多数B淋巴细胞是由造血干细胞（HSC）在骨髓中产生的，造血干细胞在胚胎发生的第10.5-11天首次出现在胎儿的主动脉-性腺-中肾区域。关于胚胎发生过程中B细胞发育的了解相对较少。例如，哪些特定的胚胎组织参与了B淋巴细胞生成，以及造血分化是否偏向胚胎中的特定B细胞亚群，这一问题仍未得到解答，因为在胚胎发生过程中系统循环就开始了，这可能导致多种细胞混合在一起。网站。我们证明，使用缺乏系统性血液循环的Ncx1〜（-/-）小鼠，E9卵黄囊（YS）和胚内副主动脉扁平瘤（P-Sp）组织独立产生AA4.1 * CD19 + B220〜（io-neg）B祖细胞在移植后优先分化为先天B-1型和边缘区（MZ）B细胞，但不分化为B-2细胞。我们还进一步证明了这些B-1祖细胞直接来自YS和P-Sp造血内皮。这些结果记录了可用于在E11处播种胎儿肝脏的先天B淋巴细胞祖细胞的初始波。这些研究的结果扩展了我们对造血内皮上位点的了解，这些位点指定了B-2和BZ细胞以外的独特B-1和MZ细胞命运，并且在发育过程中与HSC起源无关。

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    Momoko Yoshimoto; Encamacion Montecino-Rodriguez; Michael J. Ferkowicz; Prashanth Porayette; W. Christopher Shelley; Simon J. Conway; Kenneth Dorshkind; Mervin C. Yoder;

  • 作者单位

    Department of Pediatrics, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202;

    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095;

    Department of Pediatrics, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202;

    Department of Pediatrics, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202;

    Department of Pediatrics, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202;

    Department of Pediatrics, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202;

    Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA 90095;

    Department of Pediatrics, Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, IN 46202;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    B-1 cell; marginal zone B cell; OP9 stromal cells; hematopoiesis;

    机译：B-1细胞;边缘区B细胞;OP9基质细胞;造血作用;

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