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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly
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Identification of the meiotic life cycle stage of Trypanosoma brucei in the tsetse fly

机译：采采蝇中布鲁氏锥虫减数分裂生命周期阶段的鉴定

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摘要

Elucidating the mechanism of genetic exchange is fundamental for understanding how genes for such traits as virulence, disease phenotype, and drug resistance are transferred between pathogen strains. Genetic exchange occurs in the parasitic protists Trypanosoma brucei, T. cruzi, and Leishmania major, but the precise cellular mechanisms are unknown, because the process has not been observed directly. Here we exploit the identification of homologs of meiotic genes in the T. brucei genome and demonstrate that three functionally distinct, meiosis-specific proteins are expressed in the nucleus of a single specific cell type, defining a previously unde-scribed developmental stage occurring within the tsetse fly salivary gland. Expression occurs in clonal and mixed infections, indicating that the meiotic program is an intrinsic but hitherto cryptic part of the developmental cycle of trypanosomes. In experimental crosses, expression of meiosis-specific proteins usually occurred before cell fusion. This is evidence of conventional meiotic division in an excavate protist, and the functional conservation of the meiotic machinery in these divergent organisms underlines the ubiquity and basal evolution of meiosis in eukaryotes.
机译：阐明遗传交换的机制对于理解如何在病原体菌株之间转移毒力，疾病表型和耐药性等性状的基因至关重要。遗传交换发生在寄生虫原生生物Trypanosoma brucei，T。cruzi和利什曼原虫中，但是确切的细胞机制尚不清楚，因为尚未直接观察到该过程。在这里，我们利用鉴定布鲁氏杆菌基因组中减数分裂基因的同源物，并证明三种功能不同的减数分裂特异性蛋白在单个特定细胞类型的细胞核中表达，从而定义了先前未描述的发育阶段。采采蝇蝇唾液腺。表达在克隆和混合感染中发生，表明减数分裂程序是锥虫体发育周期的固有但迄今仍是隐秘的部分。在实验杂交中，减数分裂特异性蛋白的表达通常发生在细胞融合之前。这是在挖掘的原生生物中进行常规减数分裂的证据，在这些不同生物中减数分裂机制的功能保守性突出了真核生物中减数分裂的普遍存在和基础进化。

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    Lori Peacock; Vanessa Ferris; Reuben Sharma; Jack Sunter; Mick Bailey; Mark Carrington; Wendy Gibson;

  • 作者单位

    School of Biological Sciences, University of Bristol, Bristol BS8 1UG, United Kingdom Department of Clinical Veterinary Science, University of Bristol, Bristol BS40 7DU, United Kingdom;

    School of Biological Sciences, University of Bristol, Bristol BS8 1UG, United Kingdom Department of Clinical Veterinary Science, University of Bristol, Bristol BS40 7DU, United Kingdom;

    Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom Faculty of Veterinary Medicine, University Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;

    Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom;

    Department of Clinical Veterinary Science, University of Bristol, Bristol BS40 7DU, United Kingdom;

    Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom;

    School of Biological Sciences, University of Bristol, Bristol BS8 1UG, United Kingdom;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    African trypanosomes; Kinetoplastida; Euglenozoa; fluorescent reporter; Glossina;

    机译：非洲锥虫;运动质体;裸藻荧光记者格洛西娜;

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