...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
【24h】

Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass

机译：木质素的基因操作降低了顽固性并提高了柳枝ethanol的乙醇产量

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Switchgrass is a leading dedicated bioenergy feedstock in the United States because it is a native, high-yielding, perennial prairie grass with a broad cultivation range and low agronomic input requirements. Biomass conversion research has developed processes for production of ethanol and other biofuels, but they remain costly primarily because of the intrinsic recalcitrance of biomass. We show here that genetic modification of switchgrass can produce phenotypically normal plants that have reduced thermal-chemical (<180 ℃), enzymatic, and microbial recalcitrance. Down-regulation of the switchgrass caffeic acid O-methyltransferase gene decreases lignin content modestly, reduces the syringyhguaiacyl lignin monomer ratio, improves forage quality, and, most importantly, increases the ethanol yield by up to 38% using conventional biomass fermentation processes. The down-regulated lines require less severe pre-treatment and 300-400% lower cellulase dosages for equivalent product yields using simultaneous saccharif ication and fermentation with yeast. Furthermore, fermentation of diluted acid-pretreated transgenic switchgrass using Clostridium thermocellum with no added enzymes showed better product yields than obtained with unmodified switchgrass. Therefore, this apparent reduction in the recalcitrance of transgenic switchgrass has the potential to lower processing costs for biomass fermentation-derived fuels and chemicals significantly. Alternatively, such modified transgenic switch-grass lines should yield significantly more fermentation chemicals per hectare under identical process conditions.
机译：柳枝is是美国领先的专用生物能源原料，因为它是一种本地化，高产的多年生草原草，具有广泛的栽培范围和较低的农业投入要求。生物质转化研究已经开发出生产乙醇和其他生物燃料的方法，但由于生物质固有的顽固性，它们的成本仍然很高。我们在这里表明，柳枝genetic的基因改造可以产生表型正常的植物，这些植物的热化学（<180℃），酶和微生物的抵抗力降低。柳枝c咖啡酸O-甲基转移酶基因的下调适度降低了木质素含量，降低了丁香愈创木脂基木质素单体的比例，改善了草料质量，最重要的是，使用常规生物质发酵工艺可将乙醇产量提高38％。使用同步糖化和酵母发酵后，下调的品系不需要进行更严格的预处理，而纤维素酶的用量就降低了300-400％，以获得同等的产品产量。此外，使用未添加酶的热纤梭菌发酵稀释的酸预处理的转基因柳枝switch，与未修饰柳枝switch相比，具有更高的产品收率。因此，这种转基因柳枝the顽抗性的明显降低具有显着降低生物质发酵衍生燃料和化学品的加工成本的潜力。备选地，在相同的工艺条件下，这种改良的转基因柳枝lines品系每公顷应产生明显更多的发酵化学品。

著录项

  • 来源
  • 作者

    Chunxiang Fu; Jonathan R. Mielenz; Xirong Xiao; Yaxin Ge; Choo Y. Hamilton; Miguel Rodriguez Jr.; Fang Chen; Marcus Foston; Arthur Ragauskas; Joseph Bouton; Richard A. Dixon; Zeng-Yu Wang;

  • 作者单位

    Forage Improvement Division , The Samuel Roberts Noble Foundation, Ardmore, OK 73401;

    Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831 BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831;

    Forage Improvement Division , The Samuel Roberts Noble Foundation, Ardmore, OK 73401 BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831;

    Forage Improvement Division , The Samuel Roberts Noble Foundation, Ardmore, OK 73401;

    Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831 BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831;

    Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, 37831 BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831;

    BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831 Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK 73401;

    BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831 School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332;

    BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN, 37831 School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332;

    Forage Improvement Division , The Samuel Roberts Noble Foundation, Ardmore, OK 73401;

    Forage Improvement Division , The Samuel Roberts Noble Foundation, Ardmore, OK 73401;

    Forage Improvement Division , The Samuel Roberts Noble Foundation, Ardmore, OK 73401;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    biofuel crop; cellulosic ethanol; lignin modification; panicum virgatum;

    机译：生物燃料作物;纤维素乙醇木质素修饰紫薇;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号