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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Extrasynaptic δ-containing GABA_A receptors in the nucleus accumbens dorsomedial shell contribute to alcohol intake
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Extrasynaptic δ-containing GABA_A receptors in the nucleus accumbens dorsomedial shell contribute to alcohol intake

机译:伏隔核背壳中突触外含δ的GABA_A受体有助于酒精摄入

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摘要

Recent findings suggest that extrasynaptic δ-subunit-containing GABA_A receptors are sensitive to low-to-moderate concentrations of alcohol, raising the possibility that these receptors mediate the reinforcing effects of alcohol after consumption of one or a few drinks. We used the technique of viral-mediated RNAi to reduce expression of the GABA_A receptor δ-subunit in adult rats in localized regions of the nucleus accumbens (NAc) to test the hypothesis that δ-subunit-containing GABA_A receptors in the NAc are necessary for oral alcohol consumption. We found that knockdown of the δ-subunit in the medial shell region of the NAc, but not in the ventral or lateral shell or in the core, reduced alcohol intake. In contrast, δ-subunit knockdown in the medial shell did not affect intake of a 2% sucrose solution, suggesting that the effects of GABA_A receptor δ-subunit reduction are specific to alcohol. These results provide strong evidence that extrasynaptic δ-subunit-containing GABA_A receptors in the medial shell of the NAc are critical for the reinforcing effects of oral ethanol.
机译:最近的发现表明,突触外含δ亚基的GABA_A受体对低至中度浓度的酒精敏感,从而增加了这些受体在饮用一种或几种饮料后介导酒精增强作用的可能性。我们使用病毒介导的RNAi技术来降低成年大鼠伏伏核(NAc)局部区域中GABA_A受体δ-亚基的表达,以检验NAc中含δ-亚基的GABA_A受体对于口服酒精消耗。我们发现,在NAc的内侧壳区域而不是在腹侧壳或外侧壳或核心中,δ-亚基的敲低减少了酒精摄入。相比之下,内侧壳中的δ-亚基敲低并不影响2%蔗糖溶液的摄入,这表明GABA_A受体δ-亚基还原的作用是酒精特有的。这些结果提供了强有力的证据,表明NAc内侧壳中突触外含δ亚基的GABA_A受体对于口服乙醇的增强作用至关重要。

著录项

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  • 作者单位

    Ernest Gallo Clinic and Research Center, University of California, Emeryville, CA 94608;

    Ernest Gallo Clinic and Research Center, University of California, Emeryville, CA 94608;

    Ernest Gallo Clinic and Research Center, University of California, Emeryville, CA 94608;

    Ernest Gallo Clinic and Research Center, University of California, Emeryville, CA 94608 Department of Neurology, University of California, San Francisco, CA 94143 Wheeler Center for the Neurobiology of Addiction, University of California, San Francisco, CA 94143;

    Ernest Gallo Clinic and Research Center, University of California, Emeryville, CA 94608 Department of Neurology, University of California, San Francisco, CA 94143 Wheeler Center for the Neurobiology of Addiction, University of California, San Francisco, CA 94143;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    addiction; reward; self-administration; tonic inhibition;

    机译:瘾;奖励;自我管理;补品抑制;

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