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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Dynamics of endosomal sorting complex required for transport (ESCRT) machinery during cytokinesis and its role in abscission
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Dynamics of endosomal sorting complex required for transport (ESCRT) machinery during cytokinesis and its role in abscission

机译:胞质分裂过程中运输(ESCRT)机械所需的内体分选复合物的动力学及其在脱落中的作用

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摘要

The final stage of cytokinesis is abscission, the cutting of the narrow membrane bridge connecting two daughter cells. The endosomal sorting complex required for transport (ESCRT) machinery is required for cytokinesis, and ESCRT-III has membrane scission activity in vitro, but the role of ESCRTs in abscission has been undefined. Here, we use structured illumination microscopy and timelapse imaging to dissect the behavior of ESCRTs during abscission. Our data reveal that the ESCRT-I subunit tumor-susceptibility gene 101 (TSG101) and the ESCRT-III subunit charged multivesicular body protein 4b (CHMP4B) are sequentially recruited to the center of the intercellular bridge, forming a series of cortical rings. Late in cytokinesis, however, CHMP4B is acutely recruited to the narrow con-strictipn site where abscission occurs. The ESCRT disassembly factor vacuolar protein sorting 4 (VPS4) follows CHMP4B to this site, and cell separation occurs immediately. That arrival of ESCRT-III and VPS4 correlates both spatially and temporally with the abscission event suggests a direct role for these proteins in cytokinetic membrane abscission.
机译:细胞分裂的最后阶段是脱落,切开连接两个子细胞的窄膜桥。胞质分裂需要运输所需的内体分选复合物(ESCRT),并且ESCRT-III在体外具有膜分裂活性,但ESCRT在脱落中的作用尚未确定。在这里,我们使用结构化照明显微镜和延时摄影来解剖脱落时ESCRT的行为。我们的数据表明,ESCRT-I亚基肿瘤易感基因101(TSG101)和ESCRT-III亚基带电荷的多囊泡体蛋白4b(CHMP4B)依次募集到细胞间桥的中心,形成一系列皮质环。然而,在胞质分裂晚期,CHMP4B被急性募集到发生脱落的狭窄的狭窄收缩位点。 ESCRT分解因子液泡蛋白分选4(VPS4)跟随CHMP4B到达该位点,并立即发生细胞分离。 ESCRT-III和VPS4的到来在空间和时间上都与脱落事件相关,这表明这些蛋白质在细胞动力学膜脱落中具有直接作用。

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