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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Ergodic and nonergodic processes coexist in the plasma membrane as observed by single-molecule tracking
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Ergodic and nonergodic processes coexist in the plasma membrane as observed by single-molecule tracking

机译:单分子跟踪观察到,遍历和非遍历过程同时存在于质膜中

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摘要

Diffusion in the plasma membrane of living cells is often found to display anomalous dynamics. However, the mechanism underlying this diffusion pattern remains highly controversial. Here, we study the physical mechanism underlying Kv2.1 potassium channel anomalous dynamics using single-molecule tracking. Our analysis includes both time series of individual trajectories and ensemble averages. We show that an ergodic and a nonergodic process coexist in the plasma membrane. The ergodic process resembles a fractal structure with its origin in macromolecular crowding in the cell membrane. The nonergodic process is found to be regulated by transient binding to the actin cytoskeleton and can be accurately modeled by a continuous-time random walk. When the cell is treated with drugs that inhibit actin polymerization, the diffusion pattern of Kv2.1 channels recovers ergodicity. However, the fractal structure that induces anomalous diffusion remains unaltered. These results have direct implications on the regulation of membrane receptor trafficking and signaling.
机译:通常发现活细胞质膜中的扩散表现出异常动力学。但是,这种扩散模式的机制仍然存在很大争议。在这里,我们研究使用单分子跟踪的Kv2.1钾通道异常动力学的物理机制。我们的分析既包括单个轨迹的时间序列,也包括集合平均。我们表明,遍历和非遍历过程共存于质膜中。遍历过程类似于分形结构,其起源于细胞膜中的大分子拥挤。发现非遍历过程受肌动蛋白细胞骨架的瞬时结合所调节,并且可以通过连续时间的随机游走来精确地建模。当用抑制肌动蛋白聚合的药物处理细胞时,Kv2.1通道的扩散模式恢复了遍历性。但是,引起异常扩散的分形结构保持不变。这些结果对膜受体运输和信号传导的调节具有直接的影响。

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