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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Conserved Arabidopsis ECHIDNA protein mediates trans-Golgi-network trafficking and cell elongation
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Conserved Arabidopsis ECHIDNA protein mediates trans-Golgi-network trafficking and cell elongation

机译：保守的拟南芥ECHIDNA蛋白介导反高尔基网络的贩运和细胞伸长。

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摘要

Multiple steps of plant growth and development rely on rapid cell elongation during which secretory and endocytic trafficking via the trans-Golgi network (TGN) plays a central role. Here, we identify the ECHIDNA (ECH) protein from Arabidopsis thaliana as a TGN-localized component crucial for TGN function. ECH partially complements loss of budding yeast TVP23 function and a Populus ECH complements the Arabidopsis ech mutant, suggesting functional conservation of the genes. Compared with wild-type, the Arabidopsis ech mutant exhibits severely perturbed cell elongation as well as defects in TGN structure and function, manifested by the reduced association between Golgi bodies and TGN as well as mislocalization of several TGN-localized proteins including vac-uolar H+-ATPase subunit a1 (VHA-a1). Strikingly, ech is defective in secretory trafficking, whereas endocytosis appears unaffected in the mutant. Some aspects of the ech mutant phenotype can be phenocopied by treatment with a specific inhibitor of vacuolar H+-ATPases, concanamycin A, indicating that mislocalization of VHA-a1 may account for part of the defects in ech. Hence, ECH is an evolutionary conserved component of the TGN with a central role in TGN structure and function.
机译：植物生长和发育的多个步骤依赖于快速的细胞伸长，在此期间，通过反式高尔基体网络（TGN）进行的分泌和内吞运输起着核心作用。在这里，我们确定拟南芥的ECHIDNA（ECH）蛋白是TGN定位对TGN功能至关重要的组件。 ECH部分弥补了发芽酵母TVP23功能的丧失，而胡杨ECH弥补了拟南芥ech突变体的存在，表明该基因的功能保守。与野生型相比，拟南芥ech突变体表现出严重的细胞伸长以及TGN结构和功能缺陷，表现为高尔基体与TGN之间的结合减少，以及几种TGN定位蛋白（包括卵泡H +）的错误定位。 -ATPase亚基a1（VHA-a1）。令人惊讶的是，ech在分泌运输中是有缺陷的，而内吞作用在突变体中似乎不受影响。 ech突变表型的某些方面可以通过液泡H + -ATPase的特异性抑制剂伴刀豆球蛋白A的治疗进行表型复制，这表明VHA-a1的定位错误可能是ech缺陷的一部分。因此，ECH是TGN的进化保守组成部分，在TGN结构和功能中起着核心作用。

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    Delphine Gendre; Jaesung Oh; Yohann Boutte; Jacob G. Best; Lacey Samuels; Robert Nilsson; Tomohiro Uemura; Alan Marchant; Malcolm J. Bennett; Markus Grebe; Rishikesh P. Bhalerao;

  • 作者单位

    Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83 Umea, Sweden;

    Centre for Plant Integrative Biology, University of Nottingham, Loughborough LE12 5RD, United Kingdom;

    Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83 Umea, Sweden;

    Department of Botany, University of British Columbia, Vancouver, BC, Canada V6T 1Z4;

    Department of Botany, University of British Columbia, Vancouver, BC, Canada V6T 1Z4;

    Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83 Umea, Sweden;

    Department of Biological Sciences, University of Tokyo, Bunkyo-ku,Tokyo 113-0033, Japan;

    School of Biological Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom;

    Plant Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom;

    Umea Plant Science Centre, Department of Plant Physiology, Umea University, S-901 83 Umea, Sweden;

    Umea Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, S-901 83 Umea, Sweden;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 关键词

    secretory pathway; dwarf mutant; vacuolar defect;

    机译：分泌途径;矮突变体;液泡缺陷;

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