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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >F-box protein MAX2 has dual roles in karrikin and strigolactone signaling in Arabidopsis thaliana
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F-box protein MAX2 has dual roles in karrikin and strigolactone signaling in Arabidopsis thaliana

机译:F-box蛋白MAX2在拟南芥的karrikin和strigolactone信号传导中具有双重作用

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摘要

Smoke is an important abiotic cue for plant regeneration in postfire landscapes. Karrikins are a class of compounds discovered in smoke that promote seed germination and influence early development of many plants by an unknown mechanism. A genetic screen for karrikin-insensitive mutants in Arabidopsis thaliana revealed that karrikin signaling requires the F-box protein MAX2, which also mediates responses to the structurally-related strigolactone family of phytohormones. Karrikins and the synthetic strigolactone GR24 trigger similar effects on seed germination, seedling photomorphogenesis, and expression of a small set of genes during these developmental stages. Karrikins also repress MAX4 and IAA1 transcripts, which show negative feedback regulation by strigolactone. We demonstrate that all of these common responses are abolished in max2 mutants. Unlike strigolactones, however, karrikins do not inhibit shoot branching in Arabidopsis or pea, indicating that plants can distinguish between these signals. These results suggest that a MAX2-dependent signal transduction mechanism was adapted to mediate responses to two chemical cues with distinct roles in plant ecology and development.
机译:烟是篝火后植物再生的重要非生物线索。 Karrikins是在烟雾中发现的一类化合物,它们通过未知的机制促进种子发芽并影响许多植物的早期发育。拟南芥中对karrikin不敏感突变体的遗传筛选显示,karrikin信号传导需要F-box蛋白MAX2,该蛋白还介导对与植物激素相关的与结构相关的strigolactone家族的反应。 karrikins和合成的strigolactone GR24在这些发育阶段对种子萌发,幼苗光形态发生和少量基因表达触发相似的作用。 Karrikins还抑制MAX4和IAA1转录本,它们显示了strigolactone对负反馈的调节作用。我们证明在max2突变体中废除了所有这些常见的响应。但是,与角蛋白内酯不同,karrikins不会抑制拟南芥或豌豆中的枝条分支,这表明植物可以区分这些信号。这些结果表明,依赖于MAX2的信号转导机制适于介导对两种化学线索的响应,这些线索在植物生态学和发育中具有不同的作用。

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    Australian Research Council Centre of Excellence in Plant Energy Biology , University of Western Australia, Crawley WA 6009, Australia;

    School of Biomedical, Biomolecular and Chemical Sciences , University of Western Australia, Crawley WA 6009, Australia;

    School of Biological Sciences, University of Queensland, St. Lucia QLD 4072, Australia;

    Australian Research Council Centre of Excellence in Plant Energy Biology , University of Western Australia, Crawley WA 6009, Australia;

    School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia, Crawley WA 6009, Australia;

    School of Plant Biology, University of Western Australia, Crawley WA 6009, Australia Kings Park and Botanic Garden, West Perth WA 6005, Australia;

    School of Biological Sciences, University of Queensland, St. Lucia QLD 4072, Australia;

    School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia, Crawley WA 6009, Australia;

    Australian Research Council Centre of Excellence in Plant Energy Biology , University of Western Australia, Crawley WA 6009, Australia School of Biomedical, Biomolecular and Chemical Sciences , University of Western Australia, Crawley WA 6009, Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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