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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Enhancer-driven membrane markers for analysis of nonautonomous mechanisms reveal neuron-glia interactions in Drosophila
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Enhancer-driven membrane markers for analysis of nonautonomous mechanisms reveal neuron-glia interactions in Drosophila

机译：用于非自主机制分析的增强子驱动的膜标记物揭示了果蝇中的神经元-胶质细胞相互作用

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摘要

Extrinsic factors and the interactions of neurons with surrounding tissues are essential for almost every aspect of neuronal development. Here we describe a strategy of gene expression with an independent enhancer-driven cellular marker (GEEM) for studying roles of cell-cell interactions and extrinsic factors in the development of the Drosophila nervous system. Key to this strategy is robust expression of enhancer-driven transgenic markers in specific neurons. To this end, we have created vectors to achieve bright and even labeling of neuronal processes, easy cloning of enhancer elements, and efficient and flexible generation of transgenic animals. We provide examples of enhancer-driven membrane markers for specific neurons in both the peripheral and central nervous systems and their applications in the study of neuronal projections and connections in the Drosophila brain. We further applied GEEM to examine the wrapping of sensory neuron somas by glia during embryonic and larval stages, and neuron-glia interaction during den-drite pruning in live animals, leading to the discovery that glia play critical roles' in the severing and degradation of proximal dendrites. The GEEM paradigm should be applicable to the studies of both cell-autonomous and nonautonomous regulations of any cell type.
机译：外在因素以及神经元与周围组织的相互作用对于神经元发育的几乎每个方面都是必不可少的。在这里，我们描述了一种具有独立增强子驱动的细胞标记（GEEM）的基因表达策略，用于研究果蝇神经系统发育中的细胞与细胞相互作用和外在因素。该策略的关键是在特定神经元中稳定表达增强子驱动的转基因标记。为此，我们创建了载体来实现神经元过程的明亮均匀标记，轻松克隆增强子元件以及高效灵活地产生转基因动物。我们提供了在周围和中枢神经系统中特定神经元的增强子驱动膜标记物的实例，以及它们在果蝇大脑中神经元投射和连接研究中的应用。我们进一步应用GEEM来检查在动物和幼体阶段胶质细胞包裹感觉神经元的神经胶质，以及在活体动物的树突修剪过程中神经胶质细胞的相互作用，从而发现胶质细胞在神经元的切断和降解中起关键作用。近端树突。 GEEM范式应该适用于任何类型的小区自治和非自治规则的研究。

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  • 作者

    Chun Han; Lily Yeh Jan; Yuh-Nung Jan;

  • 作者单位

    The Howard Hughes Medical Institute, Departments of Physiology, Biochemistry, and Biophysics, University of California, San Francisco, CA 94143-0725;

    The Howard Hughes Medical Institute, Departments of Physiology, Biochemistry, and Biophysics, University of California, San Francisco, CA 94143-0725;

    The Howard Hughes Medical Institute, Departments of Physiology, Biochemistry, and Biophysics, University of California, San Francisco, CA 94143-0725;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    genetic tools; neuronal circuit; reporter;

    机译：基因工具;神经元回路记者;

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