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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Convergent transcription confers a bistable switch in Enterococcus faecalis conjugation
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Convergent transcription confers a bistable switch in Enterococcus faecalis conjugation

机译:聚合转录赋予粪肠球菌共轭双稳态转换

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摘要

Convergent gene pairs with, head-to-head configurations are widespread in both eukaryotic and prokaryotic genomes and are speculated to be involved in gene regulation. Here we present a unique mechanism of gene regulation due to convergent transcription from the antagonistic prgX/prgQ operon in Enterococcus faecalis controlling conjugative transfer of the antibiotic resistance plasmid pCF10 from donor cells to recipient cells. Using mathematical modeling and experimentation, we demonstrate that convergent transcription in the prgX/prgQ operon endows the system with the properties of a robust genetic switch through premature termination of elongating transcripts due to collisions between RNA polymerases (RNAPs) transcribing from opposite directions and antisense regulation between complementary counter-transcripts. Evidence is provided for the presence of truncated RNAs resulting from convergent transcription from both the promoters that are capable of sense-antisense interactions. A mathematical model predicts that both RNAP collision and antisense regulation are essential for a robust bistable switch behavior in the control of conjugation initiation by prgX/prgQ operons. Moreover, given that convergent transcription is conserved across species, the mechanism of coupling RNAP collision and antisense interaction is likely to have a significant regulatory role in gene expression.
机译:具有头对头配置的会聚基因对在真核和原核基因组中均很普遍,并推测参与基因调控。在这里,由于粪便肠球菌中拮抗性prgX / prgQ操纵子的融合转录,我们控制了抗生素抗性质粒pCF10从供体细胞向受体细胞的转移转移,从而提出了一种独特的基因调控机制。通过数学建模和实验,我们证明了prgX / prgQ操纵子中的会聚转录使系统具有稳健的遗传开关特性,这是由于从相反方向转录的RNA聚合酶(RNAP)之间的碰撞和反义调控导致的延长转录本的过早终止。互补的反转录本之间。提供了证据,表明存在两种截短的RNA,这些截短的RNA是由两个启动子的融合转录产生的,它们能够进行有义-反义相互作用。数学模型预测,RNAP碰撞和反义调节对于通过prgX / prgQ操纵子控制共轭引发的鲁棒双稳态开关行为都是必不可少的。此外,鉴于收敛转录在物种间是保守的,RNAP碰撞和反义相互作用的耦合机制可能在基因表达中具有重要的调控作用。

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