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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Unique scorpion toxin with a putative ancestral fold provides insight into evolution of the inhibitor cystine knot motif
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Unique scorpion toxin with a putative ancestral fold provides insight into evolution of the inhibitor cystine knot motif

机译:具有推测的祖先折叠的独特蝎毒素可洞悉抑制剂胱氨酸结基序的进化

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摘要

The three-disulfide inhibitor cystine knot (ICK) motif is a fold common to venom peptides from spiders, scorpions, and aquatic cone snails. Over a decade ago it was proposed that the ICK motif is an elaboration of an ancestral two-disulfide fold coined the disulfide-directed β-hairpin (DDH). Here we report the isolation, characterization, and structure of a novel toxin [U_1-liotoxin-Lw1a (U_1-LITX-Lw1a)] from the venom of the scorpion Liocheles waigien-sis that is the first example of a native peptide that adopts the DDH fold. U_1-LITX-Lw1a not only represents the discovery of a missing link in venom protein evolution, it is the first member of a fourth structural fold to be adopted by scorpion-venom peptides. Additionally, we show that U_1-LITX-Lw1a has potent insecticidal activity across a broad range of insect pest species, thereby providing a unique structural scaffold for bioinsecticide development.
机译:三二硫键抑制剂胱氨酸结(ICK)基序是蜘蛛,蝎子和水生蜗牛的毒液肽的常见折叠。十多年前,有人提出ICK基序是对二硫键导向的β-发夹结构(DDH)的祖先二-二硫键折叠的详细说明。在这里,我们从蝎子里氏鞭毛虫(Liocheles waigien-sis)的毒液中报告了一种新型毒素[U_1-liotoxin-Lw1a(U_1-LITX-Lw1a)]的分离,表征和结构,这是采用该肽的天然肽的第一个实例DDH折叠。 U_1-LITX-Lw1a不仅代表了在毒液蛋白进化过程中缺失链接的发现,而且还是蝎毒液肽所采用的第四种结构折叠的第一个成员。此外,我们表明,U_1-LITX-Lw1a在广泛的害虫种类上具有强大的杀虫活性,从而为生物杀虫剂的开发提供了独特的结构支架。

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  • 作者单位

    Institute for Molecular Bioscience, University of Queensland, St Lucia QLD 4072, Australia;

    School of Chemistry and Molecular Biosciences, University of Queensland, St Lucia QLD 4072, Australia;

    Neurotoxin Research Group, School of Medical and Molecular Biosciences, University of Technology, Sydney NSW 2007, Australia;

    Neurotoxin Research Group, School of Medical and Molecular Biosciences, University of Technology, Sydney NSW 2007, Australia;

    Institute for Molecular Bioscience, University of Queensland, St Lucia QLD 4072, Australia;

    Institute for Molecular Bioscience, University of Queensland, St Lucia QLD 4072, Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    molecular evolution; NMR; protein structure;

    机译:分子进化NMR;蛋白质结构;

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