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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Mitochondrial complex II has a key role in mitochondrial-derived reactive oxygen species influence on plant stress gene regulation and defense
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Mitochondrial complex II has a key role in mitochondrial-derived reactive oxygen species influence on plant stress gene regulation and defense

机译：线粒体复合物II在线粒体来源的活性氧对植物胁迫基因调控的防御中起关键作用

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摘要

Mitochondria are both a source of ATP and a site of reactive oxygen species (ROS) production. However, there is little information on the sites of mitochondrial ROS (mROS) production or the biological role of such mROS in plants. We provide genetic proof that mitochondrial complex II (Complex II) of the electron transport chain contributes to localized mROS that regulates plant stress and defense responses. We identify an Arabidopsis mutant in the Complex II subunit, SDH1-1, through a screen for mutants lacking GSTF8 gene expression in response to salicylic acid (SA). GSTF8 is an early stress-responsive gene whose transcription is induced by biotic and abiotic stresses, and its expression is commonly used as a marker of early stress and defense responses. Transcriptional analysis of this mutant, disrupted in stress responses 1 (dsr1), showed that it had altered SA-mediated gene expression for specific downstream stress and defense genes, and it exhibited increased susceptibility to specific fungal and bacterial pathogens. The dsr1 mutant also showed significantly reduced succinate dehydrogenase activity. Using in vivo fluorescence assays, we demonstrated that root cell ROS production occurred primarily from mitochondria and was lower in the mutant in response to SA. In addition, leaf ROS production was lower in the mutant after avirulent bacterial infection. This mutation, in a conserved region of SDH1-1, is a unique plant mitochondrial mutant that exhibits phenotypes associated with lowered mROS production. It provides critical insights into Complex II function with implications for understanding Complex ll's role in mitochondrial diseases across eukaryotes.
机译：线粒体既是ATP的来源，也是活性氧（ROS）产生的场所。但是，关于线粒体ROS（mROS）产生的位点或此类mROS在植物中的生物学作用的信息很少。我们提供了遗传证据证明电子传输链的线粒体复合体II（复合体II）有助于调节植物胁迫和防御反应的局部mROS。我们通过筛查缺少响应水杨酸（SA）的GSTF8基因表达的突变体，在复合体II亚基SDH1-1中鉴定出一个拟南芥突变体。 GSTF8是一种早期应激反应基因，其转录是由生物和非生物胁迫诱导的，其表达通常用作早期应激和防御反应的标记。该突变体的转录分析在应激反应1（dsr1）中被破坏，表明它已经改变了SA介导的特定下游应激和防御基因的基因表达，并且对特定的真菌和细菌病原体表现出更高的敏感性。 dsr1突变体还显示出琥珀酸脱氢酶活性明显降低。使用体内荧光测定法，我们证明了根细胞ROS的产生主要来自线粒体，并且在响应SA的突变体中较低。另外，在无毒细菌感染后，突变体中的叶片ROS产生较低。在SDH1-1的保守区域中，此突变是独特的植物线粒体突变体，表现出与降低的mROS产生相关的表型。它提供了对复合物II功能的批判性见解，对理解复合物II在真核生物线粒体疾病中的作用具有重要意义。

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    Cynthia Gleason; Shaobai Huang; Louise F. Thatcher; Rhonda C. Foley; Carol R. Anderson; Adam J. Carroll; A. Harvey Millar; Karam B. Singh;

  • 作者单位

    Commonwealth Scientific and Industrial Research Organisation (CSIRO) Plant Industry, Wembley, WA 6913, Australia;

    Australian Research Council (ARC) Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA 6009, Australia;

    Commonwealth Scientific and Industrial Research Organisation (CSIRO) Plant Industry, Wembley, WA 6913, Australia,Queensland Bioscience Precinct, CSIRO Plant Industry, St Lucia, QLD 4067, Australia;

    Commonwealth Scientific and Industrial Research Organisation (CSIRO) Plant Industry, Wembley, WA 6913, Australia;

    Commonwealth Scientific and Industrial Research Organisation (CSIRO) Plant Industry, Wembley, WA 6913, Australia;

    ARC Centre of Excellence in Plant Energy Biology, Australian National University, Canberra, ACT 2600, Australia;

    Australian Research Council (ARC) Centre of Excellence in Plant Energy Biology, University of Western Australia, Crawley, WA 6009, Australia;

    Commonwealth Scientific and Industrial Research Organisation (CSIRO) Plant Industry, Wembley, WA 6913, Australia,University of Western Australia Institute of Agriculture, University of Western Australia, Crawley, WA 6009, Australia;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    plant defense; respiration; pseudomonas syringae; rhizoctonia solani;

    机译：植物防御呼吸;丁香假单胞菌枯萎病;

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