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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Mapping the regulon of Vibrio cholerae ferric uptake regulator expands its known network of gene regulation
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Mapping the regulon of Vibrio cholerae ferric uptake regulator expands its known network of gene regulation

机译：绘制霍乱弧菌铁吸收调节剂的区域图谱可扩展其已知的基因调控网络

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摘要

CHIP coupled with next-generation sequencing (ChlP-seq) has revolutionized whole-genome mapping of DNA-binding protein sites. Although ChlP-seq rapidly gained support in eukaryotic systems, it remains underused in the mapping of bacterial transcriptional regulator-binding sites. Using the virulence-required iron-responsive ferric uptake regulator (Fur), we report a simple, broadly applicable ChlP-seq method in the pathogen Vibrio cholerae. Combining our ChlP-seq results with available microarray data, we clarify direct and indirect Fur regulation of known iron-responsive genes. We validate a subset of Fur-binding sites in vivo and show a common motif present in all Fur ChlP-seq peaks that has enhanced binding affinity for purified V. cholerae Fur. Further analysis shows that V. cholerae Fur directly regulates several additional genes associated with Fur-binding sites, expanding the role of this transcription factor into the regulation of ribosome formation, additional transport functions, and unique sRNAs.
机译：CHIP与下一代测序（ChlP-seq）结合，彻底改变了DNA结合蛋白位点的全基因组图谱。尽管ChlP-seq在真核系统中迅速获得支持，但在细菌转录调节因子结合位点的定位中仍未得到充分利用。使用需要毒力的铁反应性铁吸收调节剂（Fur），我们报告了一种简单，广泛适用的ChlP-seq方法，用于致病性霍乱弧菌。结合我们的ChlP-seq结果与可用的微阵列数据，我们阐明了已知铁反应基因的直接和间接Fur调控。我们验证了体内的Fur结合位点的子集，并显示了在所有Fur ChlP-seq峰中存在的共有基序，该基序对纯化的霍乱弧菌Fur具有增强的结合亲和力。进一步的分析表明，霍乱弧菌Fur直接调节与Fur结合位点相关的几个其他基因，从而将该转录因子的作用扩展到核糖体形成，其他转运功能和独特sRNA的调节中。

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