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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >PolyU tail of rho-independent terminator of bacterial small RNAs is essential for Hfq action
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PolyU tail of rho-independent terminator of bacterial small RNAs is essential for Hfq action

机译：细菌小RNA的rho非依赖性终止子的PolyU尾对于Hfq动作至关重要

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摘要

Major bacterial small RNAs (sRNAs) regulate the translation and stability of target mRNAs through base pairing with the help oi the RNA chaperone Hfq. The Hfq-dependent sRNAs consist of three basic elements, mRNA base-pairing region, Hfq-binding site, anc rho-independent terminator. Although the base-pairing region and the terminator are well documented in many sRNAs, the Hfq-bind ing site is less well-defined except that Hfq binds RNA with a pre ference for AU-rich sequences. Here, we performed mutational anc biochemical studies to define the sRNA site required for Hfq actior using SgrS as a model sRNA. We found that shortening terminatoi polyU tail eliminates the ability of SgrS to bind to Hfq and to silence ptsG mRNA. We also demonstrate that the SgrS terminator can be replaced with any foreign rho-independent terminators possessing a polyU tail longer than 8 without losing the ability to silence ptsC mRNA in an Hfq-dependent manner. Moreover, we found thai shortening the terminator polyU tail of several other sRNAs alsc eliminates the ability to bind to Hfq and to regulate target mRNAs We conclude that the polyU tail of sRNAs is essential for Hfq actior in general. The data also indicate that the terminator polyU tai plays a role in Hfq-dependent stabilization of sRNAs.
机译：主要细菌小RNA（sRNA）在RNA伴侣Hfq的帮助下通过碱基配对调节靶mRNA的翻译和稳定性。 Hfq依赖的sRNAs由三个基本元素组成，即mRNA碱基配对区，Hfq结合位点和不依赖于rho的终止子。尽管碱基对区域和终止子在许多sRNA中都有很好的文献记载，但Hfq结合位点的定义不太明确，除了Hfq优先结合富含AU序列的RNA。在这里，我们进行了突变anc生化研究，以使用SgrS作为模型sRNA来定义Hfq actior所需的sRNA位点。我们发现，缩短末端的polyU尾巴可消除SgrS与Hfq结合并沉默ptsG mRNA的能力。我们还证明，SgrS终止子可以被任何polyU尾部长于8的外源rho独立的终止子取代，而不会丧失以Hfq依赖的方式沉默ptsC mRNA的能力。此外，我们发现缩短其他几个sRNA的终止子polyU尾也消除了与Hfq结合并调节靶标mRNA的能力。我们得出结论，总的来说，sRNA的polyU尾对于Hfq actor是必不可少的。数据还表明，终止子polyU tai在依赖Hfq的sRNA稳定中起作用。

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  • 作者

    Hironori Otaka; Hirokazu Ishikawa; Teppei Morita; Hiroji Aiba;

  • 作者单位

    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, Japan;

    Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa, Nagoya 464-8602, Japan;

    Faculty of Pharmaceutical Sciences, Suzuka University of Medical Sciences, Suzuka, Mie, 513-0816, Japan;

    Faculty of Pharmaceutical Sciences, Suzuka University of Medical Sciences, Suzuka, Mie, 513-0816, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    riboregulation; rna 3' end; rnase e; ryhb;

    机译：Ribore Guachion;r na 3'en d;r nase e;ryhb;

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